| 越橘反转录转座子插入多态性分子标记开发及品种鉴别 |
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| 引用本文： | 吴志娟,方茜,李永强,陈文荣,宗宇,郭卫东.越橘反转录转座子插入多态性分子标记开发及品种鉴别[J].园艺学报,2018,45(4):753-763. |
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| 作者姓名： | 吴志娟 方茜 李永强 陈文荣 宗宇 郭卫东 |
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| 作者单位： | 浙江师范大学化学与生命科学学院，浙江省特色经济植物生物技术研究重点实验室，浙江金华 321004 |
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| 基金项目： | 浙江省自然科学基金项目（LQ16C150001）；浙江省科技厅重点研发计划项目（2018C02007）；浙江省农业新品种选育重大科技专项（2016C02052-9）；浙江省“重中之重”学科“生物学”开放基金项目 |
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| 摘 要： | 从蔓越橘（Vaccinium macrocarpon Ait.）品种‘Ben Lear’基因组序列中发掘了398 014 bp的长末端重复序列反转录转座子（LTR-RT），占整个基因组序列的11.17%；鉴别出228条Ty1-Copia家族成员和137条Ty3-Gypsy家族成员。以45个越橘（Vaccinium spp.）品种和1个蔓越橘品种为材料，从53对基于LTR-RT插入位点开发的引物中筛选出17对多态性引物，建立了越橘品种的LTR-RT插入多态性（RBIP）分子标记。使用RBIP标记对46个品种进行了亲缘关系分析和分子身份证构建，其中多态性最高的位点是Vmrg1，最低的位点是Vmrg14。其PCR产物的大小变化范围为200 ~ 463 bp，最小长度片段和最大长度片段分别出现在位点Vmrc19和Vmrc3。46个品种基于遗传距离聚为6组，组Ⅰ包括3个北高丛越橘品种，蔓越橘单独聚为组Ⅵ，其他4组中北高丛越橘、南高丛越橘或兔眼越橘混杂在一起。利用17个位点组成的“1”、“0”字符串构建了45份越橘品种和1份蔓越橘品种的唯一分子身份证，为越橘品种的鉴别提供了参考。
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| 关 键 词： | 越橘 反转录转座子 分子标记 亲缘关系 品种鉴别 |
Development of Retrotransposon-based Insertion Polymorphism Molecular Marker and Cultivar Identification of Blueberry |
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| Institution: | College of Chemistry and Life Sciences，Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants，Zhejiang Normal University，Jinhua，Zhejiang 321004，China |
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| Abstract: | A total of 398 014 bp long terminal repeat retrotransposons（LTR-RT）were found in the genomic sequence of Vaccinium macrocarpon‘Ben Lear’，accounting for 11.17% of the whole genome，among which 228 Ty1-Copia family members and 137 Ty3-Gypsy family members were identified in all LTR-RTs. Fifty-three primers were developed based on LTR-RT insertion polymorphisms. Using 45 blueberry cultivars and one cranberry cultivar，17 pairs of polymorphic primers were screened and retrotransposon-based insertion polymorphism（RBIP）system of blueberry was established. Phylogenetic analyses and molecular identity of 46 germplasms were performed using RBIP markers. The locus with the highest polymorphism was Vmrg1，the lowest locus was Vmrg14. The size of PCR products varied from 200 bp to 463 bp，and the minimum and maximum length products were found at loci Vmrc19 and Vmrc3，respectively. All the cultivars were clustered into six groups in the phylogenetic tree. GroupⅠwas comprised of three northern highbush blueberries while cranberry was clustered into group Ⅵ. Other four groups included northern highbush blueberries，southern highbush blueberries and/or rabbiteye blueberries. Molecular identity of 45 blueberry cultivars and one cranberry cultivar were constructed using“1”and“0”strings obtained from 17 polymorphic loci，which provided references for identification of bluberry cultivars. |
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| Keywords: | blueberry retrotransposon molecular marker genetic relationship cultivar identification |
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