| ‘中油桃9号’及其黄肉突变体花和叶片中类胡萝卜素代谢及相关基因的时空表达 |
| |
| 引用本文： | 朱运钦,曾文芳,牛良,蔡祖国,鲁振华,潘磊,崔国朝,王志强.‘中油桃9号’及其黄肉突变体花和叶片中类胡萝卜素代谢及相关基因的时空表达[J].园艺学报,2018,45(10):1869-1875. |
| |
| 作者姓名： | 朱运钦 曾文芳 牛良 蔡祖国 鲁振华 潘磊 崔国朝 王志强 |
| |
| 作者单位： | 1中国农业科学院郑州果树研究所，郑州 450009；2河南农业职业学院，河南中牟 451450；3河南科技学院，河南新乡453003 |
| |
| 基金项目： | 中国农业科学院科技创新工程专项经费项目（CAAS-ASTIP-2018-ZFRI）；国家科技支撑计划项目（2014BAD16B04）；河南省现代农业产业技术体系建设专项资金项目（Z2014-11-01） |
| |
| 摘 要： | 以‘中油桃9号’及其黄肉芽变的花和叶片为试材,采用HPLC法对类胡萝卜素的积累水平进行定性和定量分析,实时荧光定量PCR法对类胡萝卜素代谢关键基因的表达水平进行分析。结果表明:在花萼中,黄肉突变体的总类胡萝卜素水平为‘中油桃9号’的5.7倍,其中的β–胡萝卜素为‘中油桃9号’的19.6倍;除DXS和LCY-E外,突变体花萼中无论是合成类胡萝卜素的基因,还是降解类胡萝卜素基因的表达水平均显著高于‘中油桃9号’。在花瓣中,两个材料的类胡萝卜素总量无显著差异,但各类胡萝卜素成分含量存在显著差异;突变体DXS、HDR、PDS、LCY-E、CHY-B和CCD1的表达量显著高于‘中油桃9号’,ZDS、NCED1和CCD4的表达量显著低于‘中油桃9号’。在叶片中,‘中油桃9号’和黄肉突变体均以β–胡萝卜素、紫黄质、9–顺式–紫黄质和叶黄质为主,幼果期时两个材料的类胡萝卜素总量无显著差异,果实成熟后突变体的类胡萝卜素总量呈上升趋势,显著高于‘中油桃9号’;自花后55～80 d,仅CCD4的表达模式与类胡萝卜素的积累一致,因而认为CCD4的表达差异是两个材料后期叶片类胡萝卜素含量差异的主要原因。
|
| 关 键 词： | 油桃 芽变 白肉 黄肉 类胡萝卜素 基因 |
Temporal and Spatial Expression of Carotenoid Metabolism and Relative Genes in Flowers and Leaves of CN9 Nectarine and Its Yellow-fleshed Mutant |
| |
| ZHU Yunqin,ZENG Wenfang,NIU Liang,CAI Zuguo,LU Zhenhua,PAN Lei,CUI Guochao,WANG Zhiqiang,.Temporal and Spatial Expression of Carotenoid Metabolism and Relative Genes in Flowers and Leaves of CN9 Nectarine and Its Yellow-fleshed Mutant[J].Acta Horticulturae Sinica,2018,45(10):1869-1875. |
| |
| Authors: | ZHU Yunqin ZENG Wenfang NIU Liang CAI Zuguo LU Zhenhua PAN Lei CUI Guochao WANG Zhiqiang |
| |
| Institution: | 1. The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China;
2. Shunde Hospital Affiliated to Southern Medical University, Foshan 528330, China |
| |
| Abstract: | Two nectarine cultivars，CN9（white-fleshed）and its yellow-fleshed mutant CN9Y，were used as materials to investigate carotenoids metabolism and genes expression. Qualitative and quantitative levels of carotenoids were analyzed by using HPLC，the expression of genes related to carotenoid accumulation were detected via real-time qPCR method. The results showed that the total carotenoid and β-carotene content of CN9Y nectarine were 5.7 and 19.6 times higher than that of CN9 respectively in calyxes. Carotenoid metabolism genes of both biosynthesis and degradation showed significant higher expression level in CN9Y calyxes than in CN9，except DXS and LCY-E. In petals，the total carotenoid content was similar between CN9 and CN9Y，but their carotenoid composition contents were different. The gene expression levels of DXS，HDR，PDS，LCY-E，CHY-B and CCD1 were higher in CN9Y petals than in CN9，but ZDS，NCED1 and CCD4 were lower in CN9Y than in CN9. In leaves，the carotenoid compositions of CN9 and CN9Y were all dominated by β-carotene，violaxanthin，cis-9-violaxanthin and lutein，the total carotenoid content was similar between CN9 and CN9Y during early fruit development stage，but CN9Y obviously increased after fruits matured，however，CN9 was still stabilized. During 55–80 d after flowering，only the expression level of CCD4 was in accordance with carotenoid accumulation，it is suggested that the difference of CCD4 expression level may lead to the difference of carotenoid accumulation in leaves between the two materials in later stage. |
| |
| Keywords: | nectarine mutant white-flesh yellow-flesh carotenoid gene |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
