| 苹果CR4基因家族鉴定与表达分析 |
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| 引用本文： | 吕前前,刘钰玺,李静轩,马宗桓,姜雪峰,王宝林,毛娟,褚明宇,陈佰鸿,左存武.苹果CR4基因家族鉴定与表达分析[J].园艺学报,2018,45(10):2019-2029. |
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| 作者姓名： | 吕前前 刘钰玺 李静轩 马宗桓 姜雪峰 王宝林 毛娟 褚明宇 陈佰鸿 左存武 |
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| 作者单位： | 甘肃农业大学园艺学院，兰州 730070 |
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| 基金项目： | 甘肃农业大学学生科研训练计划SRTP项目（20171028）；国家自然科学基金项目（31860545；31501728）；甘肃省科技重大专项（18ZD2NA006）；甘肃农业大学引进人才专项（GSAU-RCZX201712） |
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| 摘 要： | 以RCC1（PF00415）、TNFR_c6（PF00020）和Pkinase（PF00069）保守域全蛋白序列为种子序列，在苹果全基因组范围比对分析了CRINKLY4（CR4）家族成员，对其理化性质、进化关系、染色体分布等进行了分析；基于qPCR分析了CR4基因在苹果腐烂病发生和低温胁迫过程中的表达情况。通过分析，共获得8个苹果CR4基因，其氨基酸序列大小介于668 ~ 1 690，分子量介于71.73 ~ 187.61 kD，等电点介于5.69 ~ 8.66，主要位于质膜；根据进化分析将其分为两个亚组。PCR表达分析结果表明，CR4基因在苹果不同组织和品种间存在不同的表达模式。分别有8个和6个CR4基因在苹果花芽感受低温和接种腐烂病菌后至少在1个时间点发生差异表达。其中，MD03G1068800在花芽感受低温后上调达25倍以上，而MD00G1166500和MD01G1153100在腐烂病发生后上调达150倍以上，以上3个CR4基因可作为后续功能研究的候选基因。
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| 关 键 词： | 苹果 CR4 生物信息学分析 低温 腐烂病 表达 |
Identification and Expression Analysis of CR4 in Apple |
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| Lü Qianqian,LIU Yuxi,LI Jingxuan,MA Zonghuan,JIANG Xuefeng,WANG Baolin,MAO Juan,CHU Mingyu,CHEN Baihong,ZUO Cunwu.Identification and Expression Analysis of CR4 in Apple[J].Acta Horticulturae Sinica,2018,45(10):2019-2029. |
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| Authors: | Lü Qianqian LIU Yuxi LI Jingxuan MA Zonghuan JIANG Xuefeng WANG Baolin MAO Juan CHU Mingyu CHEN Baihong ZUO Cunwu |
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| Institution: | Department of Horticulture，Gansu Agricultural University，Lanzhou 730070，China |
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| Abstract: | As conserved sequences of Arabidopsis CR4 protein，RCC1（PF00415），TNFR_c6 （PF00020）and Pkinase（PF00069）domains were used to search for the receptor like kinase CRINKLY4（CR4）genes in apple. The physical and chemical characteristics，evolutionary relationship and chromosomal distribution were analyzed for all of the identified CR4 genes. Additionally，tissue-specific expression profiles of CR4s were examined. Stress-specific expression of CR4s was determined in flower buds treated with lower temperature and trunks infected with Valsa mali. In total，eight CR4 genes were identified in the apple（Malus × domestica Borkh.）genome. The size（amino acid，AA），molecular weight and isoelectric point of apple CR4s varied from 668 to 1 690，from 71.73 to 187.61 kD and from 5.69 to 8.66，respectively. The expressed proteins were mainly sublocalized at plasma membrane（PM）. According to the phylogenetic analysis，apple CR4s could be divided into two sub-groups. Gene expression analysis showed that CR4s were distinctly expressed in different tissues and varieties. In addition，expression levels of eight and six CR4s were significantly changed at least in one time point when the flower bud under low temperature or the trunk was infected with Valsa mali，respectively. Among these，expression of MD03G1068800 was up-regulated more than 25 folds after the bud suffering low temperature，while that of MD00G1166500 and MD01G1153100 was up-regulated over 150 folds by Valsa mali，suggesting that these three CR4s can serve as candidate genes for further functional charaterization. This study sheds new light on future functional research of apple CR4s and provides new targets for stress-control and disease resistance regulation in apple industry. |
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| Keywords: | apple CR4 bioinformatic analysis low temperature apple canker expression |
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