| 转香樟CcCBFs烟草的抗逆性分析 |
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| 引用本文： | 王建格,周婵,张佳佳,朱逢玲,焦晓琳,杜丽.转香樟CcCBFs烟草的抗逆性分析[J].园艺学报,2018,45(10):1979-1988. |
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| 作者姓名： | 王建格 周婵 张佳佳 朱逢玲 焦晓琳 杜丽 |
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| 作者单位： | 南阳师范学院生命科学与技术学院，河南南阳 473000 |
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| 基金项目： | 国家自然科学基金项目（31100511）；南阳师范学院STP项目（2016001） |
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| 摘 要： | 为探究香樟CBF基因(CcCBFa、CcCBFb和CcCBFc)增强植株非生物胁迫抗性的功能,对获得的T1代转基因烟草,分别进行300 mmol·L~(-1)甘露醇模拟干旱、300 mmol·L~(-1) NaCl溶液模拟高盐和4℃模拟低温胁迫处理,测定其丙二醛、脯氨酸和可溶性糖含量,探讨其抗逆性。结果表明:CcCBFb和CcCBFc可显著增强烟草抗旱性;CcCBFa和CcCBFc可显著增强烟草抗盐性;CcCBFc可显著增强烟草抗寒性。CcCBFc可以提高转基因烟草抗旱、抗盐、抗寒能力。
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| 关 键 词： | 香樟 CBF基因 烟草 非生物胁迫 抗逆性 |
Analysis of Stress-resistance of CcCBFs from Cinnamomum camphora Transgenic Tobacco |
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| WANG Jiange,ZHOU Chan,ZHANG Jiajia,ZHU Fengling,JIAO Xiaolin,DU Li.Analysis of Stress-resistance of CcCBFs from Cinnamomum camphora Transgenic Tobacco[J].Acta Horticulturae Sinica,2018,45(10):1979-1988. |
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| Authors: | WANG Jiange ZHOU Chan ZHANG Jiajia ZHU Fengling JIAO Xiaolin DU Li |
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| Institution: | School of Life Science and Technology，Nangyang Normal University，Nanyang，Henan 473000，China |
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| Abstract: | In order to explore the function of three CBF genes from Cinnamomum camphora （CcCBFa，CcCBFb and CcCBFc）enhancing plant abiotic stress resistance，the obtained T1 generation of positive transgenic tobacco was subjected to stress treatment under 300 mmol · L-1 mannitol，300 mmol · L-1 NaCl solution and 4 ℃ to simulate drought，high salt and low temperature，respectively. The physiological indexes，such as proline，soluble sugar and malondialdehyde，were measured to reflect the resistance characteristics of transgenic plants. The results showed that CcCBFb and CcCBFc could significantly enhance tobacco drought resistance；CcCBFa and CcCBFc significantly enhanced the salt tolerance of tobacco；CcCBFc significantly enhanced cold tolerance of tobacco. CcCBFc could improve the ability of drought-resistance，salt-tolerance and cold-resistance of transgenic tobacco. |
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| Keywords: | Cinnamomum camphora CBF tobacco abiotic stress stress resistance |
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