军牧1号白猪、杜洛克猪、西藏小型猪和大白猪ESR、FSHβ、EGF基因的多态性分布 |
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引用本文: | 李文静,印崇,高峰,白春艳,孙博兴,邢沈阳.军牧1号白猪、杜洛克猪、西藏小型猪和大白猪ESR、FSHβ、EGF基因的多态性分布[J].兽医大学学报,2013(11):1778-1782. |
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作者姓名: | 李文静 印崇 高峰 白春艳 孙博兴 邢沈阳 |
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作者单位: | 吉林大学农学部动物科学学院,吉林长春130062 |
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基金项目: | 吉林省科技厅农业处项目(20120224);长春市科技局农业处项目(2012231);吉林大学本科生创新实验项耳(2011C81323) |
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摘 要: | 摘要:ESR、FSHβ和EGF基因与猪繁殖性状密切相关。为检测军牧1号白猪、西藏小型猪、杜洛克猪和失白猪ESR、FSHβ和EGF基因的多态性分布情况,采用PCR和PCRRFLP的方法对61头军牧1号白猪,51头杜洛克猪,51头西藏小型猪和69头大白猪的ESR、FSHβ和EGF基因多态性进行了检测。结果显示,对于各个基因的优势等位基因,在军牧1号白猪群体中,ESR基因位点A等位基因频率为0.6393,FsHp基因的13等位基因频率高达0.9098,EGF基因的A等位基因频率仅有0.0164杜洛克猪群体中,ESR基因A等位基因频率为0.6078,FSHβ基因的B等位基因频率为0.8235,而EGF基因的八等位基因频率仅为0.0297;西藏小型猪群体中,ESR基因A等位基因频率是0.4608,FSHβ基因B等位基因频率仅为0.0687,H;F基因A等位基因频率为o.1961;大白猪群体中。ESR基因位点有频率为0.5000的有害A等位基因,而FSHβ基因和EGF基因的13等位基因频率则分别为0.8013和0.7391。所有基因型分布均符合哈代温伯格平衡(P〉0.05)。
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关 键 词: | 猪 ESR基因 FSH8基因 EGF基因 |
The polymorphism distribution of gene ESR, FSHβ, and EGF in Junmu 1 white pigs,Durocs,Tibet mini-pigs and Large White |
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Authors: | LI Wen-jing YIN Chong GAO Feng BAI Chun-yan SUN Bo-xing XING Shen-yang |
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Institution: | (College of Animal Sciencs , J ilin University, Chang chun 130062, China) |
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Abstract: | ESR,FSHβand EG-F genes are correlated with reproduction traits in swine. In order to analyze the polymorphism distribution of ESR,FSHi3,and EGF genes,this study detected the gen- otype of these genes in 61 Junmu 1 white pigs,51 Durocs,51 Tibet mini pigs and 69 Large White with PCR and PCR-RFI.P. The result showed that for the advantageous allele,in Junrnu 1 white pigs,the allelic frequency of A of ESR gene is 0. 639 3,tA of FSHβ 0. 909 8,A of EGF 0. 016 4. In Durocs,A of ESR is 0. 607 8 ,B of FSHβ 0. 823 5,and A of EGF 0. 029 7. For Tibet mini-pigs,A of ESR is 0. 460 8,and B of FSHβ 0. 909 8,A of EGF 0. 196 1. In Large White,A of ESR is 0. 500 0,B of FSHβ and EGF 0. 801 3 and 0.7,'39 1 ,respectively. The distribution of all genes fit in with Har- dy Weinberg equilibrium (P〈0.05). |
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Keywords: | Swine ESR gene FSHβ gene EGF gene |
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