| 河北兔场皮肤病原真菌rDNA-ITS序列分析 |
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| 引用本文： | 刘彦威,柳焕章,刘建钗,刘娜,刘利强,王绥华,王慧真.河北兔场皮肤病原真菌rDNA-ITS序列分析[J].兽医大学学报,2013(9):1383-1386. |
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| 作者姓名： | 刘彦威 柳焕章 刘建钗 刘娜 刘利强 王绥华 王慧真 |
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| 作者单位： | 河北工程大学农学院动物医学重点实验室,河北邯郸056021 |
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| 基金项目： | 河北省科技支撑计划资助项目（11230405D-3）; 邯郸市科技局资助项目（1122101038.7） |
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| 摘 要： | 利用皮肤真菌核糖体内转录间隔区（ITS）序列通用引物,对采自河北省兔场的5种皮肤真菌病进行了PCR扩增,ITS区的克隆、测序、序列变异及遗传进化关系分析。经与GenBank核酸序列数据库数据比对和形态学观察结果表明：有4种真菌被鉴定,分别为须毛癣菌、多聚曲霉、球孢白僵菌和产黄青霉,1种未鉴定;不同病原菌的5.8SrDNA序列高度保守,而ITS区的变异性则较高。该研究确定ITS区序列分析可用于兔皮肤病原真菌的分离。
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| 关 键 词： | 家兔 皮肤真菌 核糖体基因 ITS 序列分析 |
Sequences analysis of rDNA ITS of rabbit dermatophytes in Hebei province |
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| Authors: | LIU Yan-wei;LIU Huan-zhang;LIU Jian-chai;LIU Na;LIU Li-qiang;WANG Sui-hua;WANG Hui-zhen |
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| Institution: | LIU Yan-wei;LIU Huan-zhang;LIU Jian-chai;LIU Na;LIU Li-qiang;WANG Sui-hua;WANG Hui-zhen;Key Laboratory of Animal Medicine,Agricultural College,Hebei University of Engineering; |
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| Abstract: | 5.8S rDNA and its flanking internal transcribed spacers（ITS）of 5 isolated strains of dermatophytes from rabbit farms in Hebei province were cloned and sequenced employing the universal primers ITS1 and ITS4.Through the blast analysis between ITS sequences and GenBank nucleic acid database and morphological observation,the results showed that the 4 strains in 5 isolated dermatophytes were preliminary identified as Trichophyton mentagrophytes Penicillium chrysogenum Beauveria bassiana and Aspergillus sydowii,respectively.One strain in 5 dermatophytes was unidentified.Alignment analysis indicated that the sequences of ITS was more variable than that of 5.8S rDNA.The results suggest that ITS sequence could be used as taxonomic evidence for rabbit dermatophytes. |
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| Keywords: | rabbit dermatophytes ribosomal gene internal transcribed spacer sequence analysis |
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