| 高铜对肉鸡原代肝细胞周期及细胞膜电位的影响 |
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| 引用本文： | 郭剑英,贾雪霞,李静涛,颜诚,李海琴,唐兆新.高铜对肉鸡原代肝细胞周期及细胞膜电位的影响[J].兽医大学学报,2013(9):1448-1450. |
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| 作者姓名： | 郭剑英 贾雪霞 李静涛 颜诚 李海琴 唐兆新 |
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| 作者单位： | [1]华南农业大学兽医学院,广东广州510642 [2]广东省兽用生物制品技术研究与应用企业重点实验室,广东肇庆510630 |
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| 基金项目： | 国家自然科学基金资助项目（30871900） |
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| 摘 要： | 通过流式细胞仪测定各组的细胞周期及细胞膜电位,研究高铜对肉鸡原代肝细胞凋亡的影响。将全量换液24h后的细胞分为空白对照组和低铜组（10μmol/L Cu2＋组）、高铜Ⅰ组（50μmol/L Cu2＋组）、高铜Ⅱ组（100μmol/L Cu2＋组）,培养48h后测定荧光值,结果表明,高铜组能显著引起静止期（G0/G1）肝细胞数增多、增殖期（S、G2＋M）肝细胞数减少、细胞膜电位下降（P〈0.05）;低铜组未出现明显变化;表明高浓度的铜能明显改变肝细胞的细胞周期及细胞膜电位。
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| 关 键 词： | 铜离子 流式细胞仪 细胞周期 细胞膜电位 凋亡 |
The effect of high copper on the cell cycle and cell membrane potential of primary hepatocyte in broilers |
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| Authors: | GUO Jian-ying;JIA Xue-xia;LI Jing-tao;YAN Cheng;LI Hai-qin;TANG Zhao-xin |
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| Institution: | GUO Jian-ying;JIA Xue-xia;LI Jing-tao;YAN Cheng;LI Hai-qin;TANG Zhao-xin;College of Veterinary Medicine,South China Agricultural University;Guangdong Veterinary Biologics Research and Application of Technology Key Laboratory of Business; |
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| Abstract: | To investigate the effect of high copper on the cell cycle and cell membrance potential of the primary hepatocyte in broiler,the cell were divided into normal control,low copper group（10 μmol/L Cu2＋ group）,high copperⅠgroup（50 μmol/L Cu2＋group）and high copperⅡgroup（100 μmol/L Cu2＋ group）after changed the medium in 24h.The cell cycle and cell membrane potential were measured at 48hby the methods of flow cytometry（FCM）.The results showed that the G0 / G1 phase was significantly increased（P0.05）,and S phase,G2 ＋M phase were significantly decreased（P0.05）in high copper group.The relative fluorescence intesities（RFI）of cell membrane potential were decreased significantly in high copper group;There was no obvious change in low copper group.In conclusion,high copper could change the cell cycle and cell membrane potential of primary hepatocyte significantly. |
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| Keywords: | copperion flow cytometry cell cycle cell membrane potential apoptotic |
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