| 鼠伤寒沙门菌SL1344株AcrpAasd缺失株的构建及生物学特性初步研究 |
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| 引用本文： | 田芳华,程相朝,张春杰,李银聚,李静,张明亮,李小康,李正,赵战勤.鼠伤寒沙门菌SL1344株AcrpAasd缺失株的构建及生物学特性初步研究[J].兽医大学学报,2013(11):1700-1706. |
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| 作者姓名： | 田芳华 程相朝 张春杰 李银聚 李静 张明亮 李小康 李正 赵战勤 |
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| 作者单位： | 河南科技大学动物科技学院,河南洛阳471003 |
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| 基金项目： | 河南省科技公关项目（112102110019）;河南科技大学研究生创新基金资助项目（CXJJYJS-Z005） |
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| 摘 要： | 构建鼠伤寒沙门菌SL1344△cr户△nsd双基因缺失突变株，并对其生物学特性进行初步研究。首先以Y7213（pREAasd）为供体菌，SL1344Acrp为受体菌进行结合转移，通过自杀性质粒介导的等位交换技术两步法筛选sI，1344的AcrpAasd双缺失株。该缺失株在有外源DAP的存在下能够正常生长，且能稳定遗传缺失的“sd基因；其血清型与亲本株SL1344Acrp及强毒株SL1344保持一致；其生化特性与亲本株基本相同，但与强毒株相比变化较明显，且生长速度明显减慢；雏鸡毒力试验结果表明其毒力较SL1344降低约10j倍。鼠伤寒沙门菌SL1344AcrpzSasd缺失株构建成功，为进一步开发以鼠伤寒沙门菌为载体的口服疫苗奠定了基础。
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| 关 键 词： | 鼠伤寒沙门菌 asd平衡致死系统 重组自杀性质粒 活疫苗载体 |
Construction and characterization of a Salmonella typhimurium SL1344AcrpAasd mu- tant strain |
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| Authors: | TIAN Fang-hua CHENG Xiang-chao ZHANG Chun-jie LI Yin-ju LI Jing ZHANG Ming-Iiang II Xiao-kang LI Zheng ZHAO Zhan-qin |
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| Institution: | (College of Animal Science and Technology, Henan Uni- versity of Science and Technology, Luoyang, Henan 471003, China) |
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| Abstract: | Construction and identification of Salmonella typhimurium SL134△ mutant strain （SL134△AcrpAasd） ,and the phenotype of SL134△△crpAasd mutant was analyzed preliminarily. Firstly,E. coli donor strain Z7213（pREAasd） was conjugated with SL134△Acrp,the recipient. The SL1344△crpAasd mutant was constructed through the two-step method introduced by the transduction of recombinant suicide plasmid. It indicated that AcrpAasd mutant can grow normal- ly when foreign DAP was supplied,and it was stabile with the recombinant Aasd gene in vitro ,the O and H antigens were identical to SL1344△crp and 8L1344 strain. The fermentation patterns and growth rate of the mutant were different from SL134△ strain,obviously,and the growth velocity of the mutant was more slow. The virulence of SL134△AcrpAasd mutant strain was 105 times lower than SL134△ strain. The SL134△AcrpAasd mutant was constructed successfully. It is con siderable that this AcrpAasd mutant could be a potential oral vaccine vector to express foreign an- tigen or act as a eukaruotic expression plasmid carrier. |
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| Keywords: | Salmonella typhimurium asd balanced-lethal host-vector system recombinant suicidevector live vaccine vector |
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