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| 鸭脂肪型脂肪酸结合蛋白基因（A-FABP）内含子1序列多态性分析 | |
| 引用本文： | 张海波,张依裕,徐琪,段修军,孙国波,陈国宏.鸭脂肪型脂肪酸结合蛋白基因（A-FABP）内含子1序列多态性分析[J].农业生物技术学报,2010,18(1):75-80. |
| 作者姓名： | 张海波 张依裕 徐琪 段修军 孙国波 陈国宏 |
| 作者单位： | 1. 扬州大学动物科学与技术学院,扬州,225009 2. 扬州大学动物科学与技术学院,扬州,225009;贵州大学动物科学学院,贵阳,550025 3. 江苏省畜牧兽医职业技术学院,泰州,225300 |
| 基金项目： | 国家科技部支撑计划重大专项，国家科技部支撑计划重点项目 |
| 摘 要： | 根据鸭A-FABPmRNA序列和鸡A-FABP基因组序列设计1对引物扩增出鸭脂肪型脂肪酸结合蛋白基因(A-FABP)内含子1序列(GenBank登录号:FJ536257),并根据扩增产物设计4对引物,利用PCR-SSCP对鸭A-FABP基因部分序列进行SNP检测,且对不同鸭群体进行群体遗传学分析.结果表明:克隆序列包含鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与鸡A-FABP基因内含子1同源性为75.1%;经SSCP检测,发现引物P1扩增片段有3处碱基突变,引物P3扩增片段有6处碱基突变,这些突变分别产生了3种和8种基因型.在P1位点樱桃谷鸭和苏牧麻鸭偏离了Hardy-Weinberg平衡(P |
| 关 键 词： | 鸭 脂肪型脂肪酸结合蛋白基因 克隆 |
Analysis of Polymorphism in Intron-1 of Adipocyte Fatty Acid-Binding Protein Gene(A-FABP) in Duck |
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| Zhang Haibo Zhang Yiyu , Xu Qi Duan Xiujun Sun Guobo Chen Guohong College of Animal Science , Technology,Yangzhou University,Yangzhou, Jiangsu Animal Husb,ry & Veterinary College,Taizhou, College of Animal Science,Guizhou University,Guiyang.Analysis of Polymorphism in Intron-1 of Adipocyte Fatty Acid-Binding Protein Gene(A-FABP) in Duck[J].Journal of Agricultural Biotechnology,2010,18(1):75-80. | |
| Authors: | Zhang Haibo Zhang Yiyu Xu Qi Duan Xiujun Sun Guobo Chen Guohong College of Animal Science Technology Yangzhou University Yangzhou Jiangsu Animal Husb ry & Veterinary College Taizhou College of Animal Science Guizhou University Guiyang |
| Institution: | Zhang Haibo 1 Zhang Yiyu 1,3 Xu Qi 1 Duan Xiujun 2 Sun Guobo 2 Chen Guohong 1* 1 College of Animal Science , Technology,Yangzhou University,Yangzhou,225009,2 Jiangsu Animal Husb,ry & Veterinary College,Taizhou,225300,3 College of Animal Science,Guizhou University,Guiyang,550025 |
| Abstract: | In this research the intron-1 sequence of adipocyte fatty acid-binding protein gene(A-FABP) was cloned(GenBank accession No.FJ536257) by a pair of primers P-in1,designed according to the sequences of A-FABP mRNA in duck and A-FABP gene in chicken,then four pairs of primers were designed according to the amplification product.The intron-1 of A-FABP gene SNPs were detected by technique of PCR-SSCP,confirmed by sequencing and to study the genetic discrepancy in different groups.The results indicated that the p... |
| Keywords: | SSCP Duck Adipocyte fatty acid-binding protein gene(A-FABP) Clone SSCP |
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