| 45S rDNA在4种百合属植物染色体上的物理定位 |
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| 引用本文： | 周树军,Jaap van Tuyl,臧德奎,夏宜平,李方.45S rDNA在4种百合属植物染色体上的物理定位[J].园艺学报,2008,35(6):859-862. |
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| 作者姓名： | 周树军 Jaap van Tuyl 臧德奎 夏宜平 李方 |
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| 作者单位： | (浙江大学农业与生物技术学院, 杭州310029；Plant Breeding, Plant Science Group, Wageningen University, P.O. Box 16, Wageningen 6700 AA, The Netherlands；3山东农业大学林学院，山东泰安271018) |
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| 基金项目： | 国家自然科学基金
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| 摘 要： | 利用荧光原位杂交的技术将45S rDNA在麝香百合(Lilium longiflorum)，柠檬色百合(L. leichtlinii), 天香百合(L. auratum)和豹纹百合(L. pardalinum)的染色体上进行了定位。结果表明：45S rDNA在这4种植物中都分布于染色体的着丝点附近，但其位点数量、所在的染色体和信号的强弱有很大的变化。其中，麝香百合和豹纹百合中各有3对染色体在着丝点附近有45S rDNA的信号，柠檬色百合和天香百合中各有4对染色体在着丝点附近有45S rDNA的信号。但是天香百合的两对中部染色体的着丝点附近都有45S rDNA的信号，柠檬色百合的两对中部染色体中只有一条有45S rDNA的信号，而麝香百合和豹纹百合中没有任何一条有45S rDNA的信号。由于这4种百合在百合属中分别属于不同组，其45S rDNA的位点数量、所在染色体和信号强弱的变化为这种分组的合理性提供了一些分子生物学的证据。
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| 关 键 词： | 百合 rDNA 荧光原位杂交(FISH) 核型 染色体 |
| 收稿时间： | 2008-01-28 |
Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium |
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| ZHOU Shu-jun,Jaap van Tuyl,ZANG De-kui,XIA Yi-ping,LI Fang.Physical Localization of 45S rDNA on the Chromosomes of 4 Species of the Genus Lilium[J].Acta Horticulturae Sinica,2008,35(6):859-862. |
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| Authors: | ZHOU Shu-jun Jaap van Tuyl ZANG De-kui XIA Yi-ping LI Fang |
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| Institution: | （College of Agriculture and Biotechnology, Zhejiang Univeristy, Hangzhou, 310029, China; Plant Breeding, Plant Science Group, Wageningen University, P.O. Box 16, Wageningen 6700 AA, The Netherlands; College of Forestry, Shandong Agricultural University, Tai'an, Shandong, 271018, China） |
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| Abstract: | 45S rDNA was mapped on the chromosomes of Lilium. longiflorum, L. leichtlinii, L. auratum and L. pardalinum of the genus Lilium using fluorescence in situ hybridization in this paper. The result showed that 45S rDNA were localized near the centromere region in these four species, but the number of the 45S rDNA sites, which chromosomes having the 45S rDNA signal, and the intensity of the 45S rDNA signal somewhat varied in the four species. Three pairs of chromosomes have the 45S rDNA signal in L. longiflorum and L. pardalinum, while four pairs of chromosomes have the 45S rDNA signal in L. leichtlinii and L. auratum. Nevertheless, the two pairs of metacentric chromosomes of L. auratum have the 45S rDNA signal, one pair of metacentric chromosomes of L. leichtlinii have the 45S rDNA signal, but no 45S rDNA signal is on any metacentric chromosome of L. longiflorum and L. pardalinum. Considering the four species belonging to different sections in Lilium, the result supplies some molecular evidence for supporting the Lilium classification. |
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| Keywords: | rDNA |
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