| 日本梨活体花柱内花粉RNA的降解 |
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| 引用本文： | 徐义流,张绍铃.日本梨活体花柱内花粉RNA的降解[J].园艺学报,2005,32(4):599-603. |
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| 作者姓名： | 徐义流 张绍铃 |
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| 作者单位： | (南京农业大学园艺学院, 南京210095) |
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| 基金项目： | 国家自然科学基金项目(30170651)，高校博士学科点专项科研基金项目(20010307012)，江苏省高技术项目(BG2002310) |
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| 摘 要： | 用32 P标记的花粉进行自花与异花授粉后, 提取含有花粉32 P-RNA的授粉花柱RNA测定放射性活度, 并将授粉花柱RNA样品进行非变性琼脂糖电泳。结果表明, 授粉后12～48 h, 自花授粉花柱RNA 中花粉32 P-RNA只占异花授粉花柱RNA中的40.7%～25.3% , 自花授粉花柱中花粉完整RNA的量特异性地减少, 表明自花花粉RNA在花柱中被特异性地降解。
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| 关 键 词： | 梨 花柱 花粉 RNA 降解 自交不亲和性 |
| 文章编号： | 0513-353X（2005）04-0599-05 |
| 收稿时间： | 2004-10-15 |
| 修稿时间： | 2004-10-152005-03-28 |
Degradation of Pollen RNA in Japanese Pear Style in Vitro |
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| Xu Yiliu,Zhang Shaoling.Degradation of Pollen RNA in Japanese Pear Style in Vitro[J].Acta Horticulturae Sinica,2005,32(4):599-603. |
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| Authors: | Xu Yiliu Zhang Shaoling |
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| Institution: | (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China) |
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| Abstract: | The RNAs were isolated from styles that were self-and cross-pollinated with ~(32)P-labelled pollens in vivo.Than radioactivity of pollinated style RNA was determined with a liquid scintillation counter,and agarose gel electrophoresis was used to test the degradation of RNA from pollinated styles.Results were that the content of pollen ~(32)P-RNA in self-pollinated style RNA is 40.7%-25.3% that of pollen ~(32)P-RNA in cross-pollinated style RNA after pollination 12-48 h,and that pollen intact RNA in self-pollinated style was specifically decreased.This indicated that pollen RNA in self-pollinated style was specifically degraded. |
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| Keywords: | Pear Style Pollen RNA Degradation Self-incompatibility |
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