| 5-azaC对萝卜茎尖DNA甲基化和开花的影响 |
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| 引用本文： | 汪炳良,李水凤,曾广文,邓俭英.5-azaC对萝卜茎尖DNA甲基化和开花的影响[J].核农学报,2005,19(4):265-268. |
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| 作者姓名： | 汪炳良 李水凤 曾广文 邓俭英 |
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| 作者单位： | [1]浙江大学园艺系,浙江杭州310029 [2]农业部园艺植物生长发育与生物技术重点开放实验室,浙江杭州310029 [3]浙江大学生命科学学院,浙江杭州310029 [4]广西农业科学院蔬菜研究中心,广西南宁530007 |
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| 基金项目： | 浙江省自然科学基金（300266） |
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| 摘 要： | 本文研究了去甲基化因子5氮杂胞苷(5azaC)对萝卜开花及幼苗茎尖DNA甲基化水平的影响。用0(对照)、0.10、0.25、0.50和1.00mmolL5azaC处理‘短叶13’萝卜种子6d,除对照外,茎尖组织DNA甲基化水平均有所降低,并与春化后茎尖组织DNA甲基化水平相当;茎尖组织DNA甲基化水平随着处理浓度的提高而下降。同时,5azaC处理明显促进春性品种萝卜‘短叶13’的开花。结果表明5azaC可以部分代替低温诱导萝卜开花。
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| 关 键 词： | 萝卜 5-azaC 花芽分化 开花 DNA甲基化 |
| 文章编号： | 1000-8551（2005）04-265-04 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005年2月28日 |
EFFECT OF 5-AZACYTOSINE ON FLOWERING AND DNA METHYLATION LEVEL OF STEM APICES IN RADISH (Raphanus sativus L.) |
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| Wang BingLiang;Li ShuiFeng;Ceng GuangWen;Deng JianYing.EFFECT OF 5-AZACYTOSINE ON FLOWERING AND DNA METHYLATION LEVEL OF STEM APICES IN RADISH (Raphanus sativus L.)[J].Acta Agriculturae Nucleatae Sinica,2005,19(4):265-268. |
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| Authors: | Wang BingLiang;Li ShuiFeng;Ceng GuangWen;Deng JianYing |
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| Abstract: | The effects of 5-azacytosine (5-azaC, a demethylation agent) on flowering and DNA methylation level of stem apices in radish were studied. Seeds of radish cultivar ‘Duan-Ye 13' were treated with 010～100 m mol/L 5-azaC for 6 days 5-azaC treatment induced DNA methylation level decrease in stem apices. The DNA methylation level in stem apices treated with 5-azaC was comparable to (even lower than) that of seedlings treated at 5℃ for 10 days. At the tested concentrations, the higher the 5-azaC concentration was, the lower the DNA methylation level in stem apices. 5-azaC treatment also promoted radish flowering, and the promotion effect was more significant in the spring radish cultivar ‘Duan-Ye 13'. Results verified that 5-azaC could partial substitute the low temperature treatment for radish vernalization. |
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| Keywords: | 5-azacytosine floral bud differentiation flowering DNA methylation |
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