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| 不同品系小鼠精子冷冻及冻融精子体外受精的研究 | |
| 引用本文： | 陆晔,唐一岷,刘丽均,谢恩,薛智谋,徐平.不同品系小鼠精子冷冻及冻融精子体外受精的研究[J].上海交通大学学报(农业科学版),2003,21(Z1):6-9. |
| 作者姓名： | 陆晔 唐一岷 刘丽均 谢恩 薛智谋 徐平 |
| 作者单位： | 1. 国家啮齿类实验动物种子中心,上海分中心,上海,201615;苏州大学,生命科学学院,苏州,215006 2. 国家啮齿类实验动物种子中心,上海分中心,上海,201615 3. 苏州大学,生命科学学院,苏州,215006 |
| 基金项目： | 国家科技基础性工作专项资金项目(2001DEA10001) |
| 摘 要： | 采用小鼠精子冷冻、冻融精子体外受精的方法,进行小鼠保种、引种、生物净化及品系标准化的可行性研究,并初步探究小鼠精子冷冻的影响因素。实验选用同一年龄5种不同品系小鼠分别进行精子冷冻、复苏,并比较各品系冷冻效果及冻融精子的体外受精(IVF)率。结果显示,冷冻后各品系小鼠精子复苏存活率约在15%～50%之间;冻融小鼠精子体外受精结果随遗传背景的不同差异显著,受精率分别为:KM小鼠68.4%、ICR小鼠30.3%、DBA/2J小鼠47.2%、C57BL/6J小鼠6.8%、BALB/cA小鼠25%。冻融精子IVF得到的胚胎可以耐受体外培养。 |
| 关 键 词： | 小鼠精子 冷冻保存 体外受精 |
| 文章编号： | 1671-9964(2003)S0-0006-04 |
| 修稿时间： | 2003年4月28日 |
Studies of in vitro Fertilization(IVF) by Cryopreserved Spermatozoa in Several Mouse Strains |
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| LU Ye,TANG Yi min,LIU Li jun,XIE En,XUE Zhi mou,XU Ping.Studies of in vitro Fertilization(IVF) by Cryopreserved Spermatozoa in Several Mouse Strains[J].Journal of Shanghai Jiaotong University (Agricultural Science),2003,21(Z1):6-9. | |
| Authors: | LU Ye TANG Yi min LIU Li jun XIE En XUE Zhi mou XU Ping |
| Institution: | LU Ye1,2,TANG Yi min1,LIU Li jun1,XIE En1,XUE Zhi mou2,XU Ping1 |
| Abstract: | Mice are the most commonly used laboratory animals, owing to the availability of large amounts of information on their genetics and biology. As a result, cryopreservation of mouse spermatozoa has recently became available in the mouse model archiving. We attempted to practise the technique of mouse sperm freezing and in vitro fertilization of frozen- thawed sperm in our laboratory. Sperm from KM,ICR,DBA/2J,mBALB/cA and C57BL/6J mouse were frozen in 18% raffinose/3% skim milk cryoprotective solution. The post- thawed sperm from all strains were evaluated for their viability and fertility by comparing them with their fresh sperm as a control. The progressive motility and fertility of sperm in each strain were affected differently after cryopreservation. The fertilization rates of the same strains oocytes inseminated with frozen- thawed spermatozoa were 68.4% ,30.3% ,47.2% ,25% and 6.8% respectively in KM,ICR,DBA/2J,BALB/cA and C57BL/6J mouse. These results indicate that cryopreservation of spermatozoa provides an effective alternative to embryos freezing and corroborate that successful cryopreservation is related to genetic background of mouse strains. |
| Keywords: | mouse spermatozoa cryopreservation in vitro fertilization |
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