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| 猪IL-15与PRRS病毒GP5基因核酸疫苗免疫效力 | |
| 引用本文： | 石娜,尹杰超,Dante Zarlenga,李广兴,任晓峰.猪IL-15与PRRS病毒GP5基因核酸疫苗免疫效力[J].东北农业大学学报,2010,41(1). |
| 作者姓名： | 石娜 尹杰超 Dante Zarlenga 李广兴 任晓峰 |
| 作者单位： | 1. 东北农业大学动物医学学院,哈尔滨,150030 2. 东北农业大学生命科学学院,哈尔滨,150030 3. 美国农业部农业研究所中心牛功能基因组和动物寄生虫病实验室,马里兰州,20705 |
| 基金项目： | 国家自然科学基金(30700590;30972937);;黑龙江省自然科学基金重点项目(ZJN0702-01) |
| 摘 要： | 通过分子克隆技术构建了表达猪白细胞介素(IL)-15及猪繁殖呼吸综合症(PRRSV)病毒GP5基因重组核酸疫苗。以小鼠为试验模型,利用猪IL-15作为分子佐剂并检测其对表达PRRS病毒GP5基因核酸疫苗的免疫增强作用。结果表明,猪IL-15与GP5试验组小鼠抗体水平和T淋巴细胞亚群数量均高于PBS对照组及GP5基因单独免疫组,表明猪IL-15对核酸疫苗可有效增强PRRS病毒GP5基因的免疫效果,证实了其佐剂效应。 |
| 关 键 词： | 猪IL-15 PRRSV GP5基因 疫苗 |
Preliminary evaluation on immunization of porcine IL-15 and PRRSV GP5 genes in mice |
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| SHI Na,YIN Jiechao,Dante Zarlenga,LI Guangxing,REN Xiaofeng.Preliminary evaluation on immunization of porcine IL-15 and PRRSV GP5 genes in mice[J].Journal of Northeast Agricultural University,2010,41(1). | |
| Authors: | SHI Na YIN Jiechao Dante Zarlenga LI Guangxing REN Xiaofeng |
| Institution: | 1.College of Veterinary Medicine;Northeast Agricultural University;Harbin 150030;China;2.College of Life Sciences;3.Bovine Functional Genomics Laboratory and Animal Parasitic Diseases Laboratory;Agricultural Research Service;US Department of Agriculture;Beltsville Maryland 20705;USA |
| Abstract: | The recombinant plasmids containing either PRRSV GP5 gene or porcine IL-15 gene were constructed by using molecular cloning techniques,and mice were used as animal models.The preliminary evaluation on the effect of IL-15 on PRRSV GP5 gene vaccination was performed through inoculating the vaccines to mice.The results showed that both antibody level,the number of T lym-phocytes,and the peripheral blood of immunized mice were higher than those of the control group.IL-15 and GP5 combined group was better than G... |
| Keywords: | porcine IL-15 PRRSV GP5 gene vaccine |
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