| 大蕉幼雄花易碎胚性愈伤组织长期继代培养及其植株再生 |
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| 引用本文： | 李哲.大蕉幼雄花易碎胚性愈伤组织长期继代培养及其植株再生[J].中国农学通报,2010,26(1):27-31. |
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| 作者姓名： | 李哲 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,国家重要热带作物工程技术研究中心,海南儋州,571737 |
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| 基金项目： | 国家自然科学基金项目"橡胶树胚性细胞悬浮培养及其植株再生的研究"，国际科技合作项目"橡胶树易碎愈伤组织长期培养植株再生和超低温保存研究"，海南省重点科技项目"橡胶树花药胚性愈伤组织的超低温保存和冻后植株再生的研究"，行业科技专项子课题"橡胶树胚性细胞悬浮培养植株再生体系建立的研究"，基本科研业务费专项资金项目"橡胶树单细胞遗传转化体系建立的研究" |
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| 摘 要： | 大蕉未成熟雄花接种到胚性愈伤组织诱导培养基中，4～5个月后可诱导出胚性愈伤组织，并可在继代培养基上长期增殖。这些继代培养了6年多的松软易碎的胚性愈伤组织转移到含有0.2 mg/L 6-BA的分化培养基中，可诱导出芽，得到了53株再生植株，这些再生植株可进一步扩大繁殖。组织学切片证明长期继代培养的愈伤组织维持了胚性的状态。
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| 关 键 词： | 甘薯 甘薯 产量 农艺性状 气象因子 关联度 |
| 收稿时间： | 2009/12/4 0:00:00 |
| 修稿时间： | 2009/12/23 0:00:00 |
Long-term Subculture and Plant Regeneration from Friable and Embryogenic Callus Induced from Inflorescences of Musa paradisiaca ABB Linn. |
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| Li Zhe.Long-term Subculture and Plant Regeneration from Friable and Embryogenic Callus Induced from Inflorescences of Musa paradisiaca ABB Linn.[J].Chinese Agricultural Science Bulletin,2010,26(1):27-31. |
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| Authors: | Li Zhe |
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| Institution: | Li Zhe (Rubber Research Institute, CATAS; State Engineering and Technology Research Center for Key Tropical Crops, Danzhou Hainan 571737) |
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| Abstract: | Immature male flowers of Musa paradisiaca ABB Linn. were inoculated on embryogenic callus induction media. Yellow granular calli emerged after 4–5 months were shown to be embryogenic calli. And they could be proliferated by long-term subculture. These friable and embryogenic calli had been subcultured for 6 years. After they were transplanted on differentiation medium containing 0.2 mg/L 6-BA, buds could be induced, and 53 regeneration plantlets had been obtained. These plantlets could be propagated further. Histological sections proved that the calli proliferated by long-term subculture maintained the embryogenic character. |
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| Keywords: | Musa paradisiaca ABB Linn friable embryogenic callus tissue culture long-term subculture plant regeneration |
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