| 禽大肠杆菌耐药标记弱毒菌株O_2(Nor~r,Chl~s)和O_(78)(Chl~r,Nor~s)的培育 |
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| 引用本文： | 黄青云,陈金顶,任涛,罗贤忠,欧守杼.禽大肠杆菌耐药标记弱毒菌株O_2(Nor~r,Chl~s)和O_(78)(Chl~r,Nor~s)的培育[J].华南农业大学学报,1997(2). |
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| 作者姓名： | 黄青云 陈金顶 任涛 罗贤忠 欧守杼 |
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| 作者单位： | 华南农业大学动物医学系 |
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| 摘 要： | 选择最常见的禽致病性大肠杆菌Ｏ２和Ｏ７８菌株，分别以常用的抗菌药物氟哌酸和氯霉素诱导，培育成遗传性稳定的耐药标记弱毒菌株Ｏ２（Ｎｏｒｒ，Ｃｈｌｓ）和Ｏ７８（Ｃｈｌｒ，Ｎｏｒｓ），为进一步运用原生质体融合技术培育Ｏ２和Ｏ７８融合双价耐药弱毒菌株打下了基础
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| 关 键 词： | 大肠杆菌 耐药菌株 弱毒菌株 |
STUDIES ON DEVELOPMENT OF DRUG-TOLERANT AND ATTENUATED O 2 (Nor r,Chl s) AND O 78 (Chl r,Nor s) STRAINS OF Escherichia coli FROM POULTRY |
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| Huang Qingyun,Chen Jinding,Ren Tao,Luo Xianzhong,Ou Shoushu.STUDIES ON DEVELOPMENT OF DRUG-TOLERANT AND ATTENUATED O 2 (Nor r,Chl s) AND O 78 (Chl r,Nor s) STRAINS OF Escherichia coli FROM POULTRY[J].Journal of South China Agricultural University,1997(2). |
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| Authors: | Huang Qingyun Chen Jinding Ren Tao Luo Xianzhong Ou Shoushu |
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| Abstract: | This paper reports the development process of drug-tolerant and attenuated Escherichia coli O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s) strains. With Escherichia coli O 2 and O 78 which are the most important serotypes of E.coli in poultry colibacillosis as parent strains, drug resistant and attenuated O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s ) strains of E.coli were induced by Norfloxacin and Chloramphenicol which are two antibacterial drugs most frequently used in veterinary practice. The essential characteristics of E.coli O 2 (Nor r,Chl s ) and O 78 (Chl r,Nor s) strains were examined. The results indicated that the drug-tolerance and the heredity of E.coli O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s) strains were stable. This achievement might be a good beginning for further developing efficient and multivalent vaccines by using spheroplast fusion technique. |
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| Keywords: | E coli drug-tolerant strain attenuated strain |
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