| 杏鲍菇高产菌株的紫外线诱变选育 |
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| 引用本文： | 况丹,叶亚建,王飞.杏鲍菇高产菌株的紫外线诱变选育[J].安徽农业科学,2010,38(16):8404-8405. |
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| 作者姓名： | 况丹 叶亚建 王飞 |
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| 作者单位： | 宜春职业技术学院食用菌研究所,江西宜春,336000;江西农业大学生物科学与工程学院,江西南昌,330045 |
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| 摘 要： | 目的]获得杏鲍菇高产菌株。方法]通过紫外诱变、单菌落分离、突变株选择、菌丝生长及出菇对比试验研究了杏鲍菇高产菌株的诱变育种技术。结果]将经紫外线照射处理的孢子悬液稀释,涂在平板培养基上,7 d后分离单菌落并两两配对。取镜检有锁状联合的菌株菌落与原始菌株做拮抗试验,得到了11株突变株。7号和11号菌株与其他菌株间有极显著差异,5号和10号菌株的生长速度显著高于原始菌株,2号菌株与对照菌株相比存在显著差异,其余菌株与原始菌株之间无显著差异。9个菌株的产量存在显著差异,其中5号和10号菌株的产量极显著高于原始菌株,分别提高了35%和34%。它们被命名为PL-5和PL-10。结论]经紫外线诱变选育的杏鲍菇菌株表现出良好的高产性能。
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| 关 键 词： | 杏鲍菇 紫外诱变 育种 |
Breeding by UV Mutation of High Yield Pleurotus eryngii Strains |
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| KUANG Dan et al.Breeding by UV Mutation of High Yield Pleurotus eryngii Strains[J].Journal of Anhui Agricultural Sciences,2010,38(16):8404-8405. |
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| Authors: | KUANG Dan |
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| Institution: | KUANG Dan et al(Institute of Edible Fungi,Yichun Vocational Technical College,Yichun,Jiangxi 336000) |
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| Abstract: | Objective] The aim was to obtain high yield Pleurotus eryngii strains.Method] The mutation breeding technology of high yield P.eryngii strains was studied through UV mutation test,isolating mono-colony,selecting mutate,mycelial growth and comparative mushroom-production test.Result] The spore suspension treated with UV irradiation was diluted and smeared on plate medium.The mono-colonies were isolated after 7 d for pair-matching.The colonies of strains with clamp connection in microscopic detection were ... |
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| Keywords: | Pleurotus eryngii UV mutation Breeding |
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