| 转Bt-pta-bar基因玉米侧翼序列分析及特异性检测方法研究 |
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| 引用本文： | 董立明,于维,李葱葱,张明,李飞武.转Bt-pta-bar基因玉米侧翼序列分析及特异性检测方法研究[J].玉米科学,2013,21(3):30-34. |
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| 作者姓名： | 董立明 于维 李葱葱 张明 李飞武 |
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| 作者单位： | 1. 吉林省农业科学院,长春,130033
2. 吉林省种子管理总站,长春,130062 |
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| 摘 要： | 采用染色体步移技术(Genome Walking)分离转Bt-pta-bar基因玉米JL937的插入位点侧翼序列，获得了615 bp的5’端边界序列。据此序列设计特异性引物，建立了JL937玉米的特异性定性PCR检测方法，可特异性地从JL937玉米中扩增出221 bp的产物。此方法具有特异性强、稳定性好的特点，适用于JL937玉米的定性检测。
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| 关 键 词： | 转基因玉米 染色体步移技术 侧翼序列 定性PCR |
| 收稿时间： | 2012/8/27 0:00:00 |
Event-specific Qualitative PCR Detection of Transgenic Maize with Bt-pta-bar Gene Based upon the 5'-transgene Integration Sequence |
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| DONG Li-ming,YU Wei,LI Cong-cong,ZHANG Ming and LI Fei-wu.Event-specific Qualitative PCR Detection of Transgenic Maize with Bt-pta-bar Gene Based upon the 5'-transgene Integration Sequence[J].Journal of Maize Sciences,2013,21(3):30-34. |
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| Authors: | DONG Li-ming YU Wei LI Cong-cong ZHANG Ming and LI Fei-wu |
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| Institution: | Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Seed Administration Station, Changchun 130062, China;Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Academy of Agricultural Sciences, Changchun 130033;Jilin Academy of Agricultural Sciences, Changchun 130033 |
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| Abstract: | The 5'-transgene integration sequence between the host plant DNA and the integrated gene construct of the GM maize JL937 was revealed by means of Genome Walking technology, and a 615 bp flanking sequence was arrived. According to this sequence, the specific PCR primers were designed, and the conventional qualitative PCR method was successfully developed. The validation results indicated that this established method was reliable, specific, and suitable for the qualitative detection of JL937 event. |
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| Keywords: | GM maize Genome Walking Integration sequence Qualitative PCR |
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