Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard |
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| Authors: | A K Mukherjee T Mohapatra A Varshney R Sharma R P Sharma |
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| Institution: | National Research Centre on Plant Biotechnology, IARI, New Delhi-110 012, India;Rajasthan Agricultural University, Bikaner, India |
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| Abstract: | Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC‐144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC‐144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0l000 and OPB061000, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes. |
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| Keywords: | Brassica juncea Albugo candida white rust resistance bulked segregant analysis molecular mapping RAPD |
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