| 三种不同给药途径建立小鼠肝硬化模型的比较 |
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| 引用本文： | 田源,邓媛媛.三种不同给药途径建立小鼠肝硬化模型的比较[J].贵州大学学报(农业与生物科学版),2012,31(5):454-456. |
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| 作者姓名： | 田源 邓媛媛 |
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| 作者单位： | 1. 贵州大学生命科学学院,贵州贵阳,550025
2. 中山大学,广东广州,100853 |
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| 基金项目： | 贵州大学自然科学青年科研基金项目 |
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| 摘 要： | 按逐步增加食用白酒浓度的方法诱导肝硬化的模型,将80只KM雄性小鼠随机分为4组,A组采用灌胃,B组采用皮下注射,C组采用腹腔注射,D组为对照组,并把20只SD大鼠随机分为皮下注射E组、对照组F组。观察各组的体重变化、死亡率和肝假小叶形成率。采用上述方法诱导7周后A～F组小鼠体重早期缓慢下降,后期缓慢增加。A组死亡5只,死亡率25%,假小叶形成率65.2%;B组死亡1只,死亡率5%,假小叶形成率92.5%;C组死亡2只,死亡率10%,假小叶形成率86.5%;E组大鼠体重早期明显下降,后期缓慢增加,死亡5只,死亡率50%,假小叶形成率66.7%;D组、F组体重明显增加,无死亡,无肝硬化形成。结果表明:小鼠皮下注射给药,逐步增加食用白酒的浓度可以缩短肝硬化形成的时间,提高形成率,降低死亡率。
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| 关 键 词： | 小鼠 肝硬化 动物模型 给药途径 |
Establishment of Mouse Model for Cirrhosis Using Three Routes of Administration |
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| TIAN Yuan
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DENG Yuan-yuan.Establishment of Mouse Model for Cirrhosis Using Three Routes of Administration[J].Journal of Mountain Agriculture & Biology,2012,31(5):454-456. |
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| Authors: | TIAN Yuan DENG Yuan-yuan |
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| Institution: | 2 ( 1. College of Life Sciences, Guizhou University, Guiyang Guizhou 550025, China; 2. Sun Yat- sen University, Guangzhou Guangdong 100855, China) |
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| Abstract: | To investigate the effects on the establishment of a mouse model for cirrhosis, a total of 80 KM male mice were divided randomly into four groups： Group A using intragastric administration （ i. g. ）, group B using subcutaneous injection （s. c. ）, group C by intraperitoneal injection （i. p. ） and group D as the con- trol. Twenty SD rats were allotted randomly into the hypodermic group （E） and the control （F）. The chan- ges of weight, the formation rate of liver cirrhosis false lobe in various groups were investigated. Induced for 7 weeks, the rate mortalily from groups A, B, C and E were 25% , 5% , 10% and 50% , respectively. The formation rate of liver cirrhosis false lobe in groups A, B, C and E were 65.2% , 92.5% , 86.5% and 66. 7% , respectively. The weight in groups D and F increased significantly, and no death or cirrhosis were observed. The result justified the cirrhotic model in mice induced by moderately increasing eatable alcohol showed a low moratality and high success rate. |
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| Keywords: | mice cirrhosis animal model rout of administration |
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