Characterization of Vitis germplasm using random amplified polymorphic DNA markers |
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| Authors: | Maria Stella Grando Luigi De Micheli Attilio Scienza |
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| Institution: | (1) Istituto Agrario di San Michele all'Adige, Via Mach 1, 38010 San Michele all'Adige, Trento, Italy;(2) Istituto di Colture Arboree, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy |
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| Abstract: | Summary An analysis of the amplification fragments polymorphism of DNA coming from different accessions of germplasm belonging to species and cultivars of the genus Vitis, was carried out using 40 primer decamers of arbitrary sequence. The RAPD profiles showed a great intraspecific diversity. In many cases a single primer produced a unique pattern for each species. A phylogram tree based upon presence/absence data of the principal DNA bands divided the species according to their geographical origins. The intraspecific polymorphism of DNA fragments was not sufficient for an unambiguous identification of Vitis vinifera cultivars but the RAPD profiles turned out to be highly reproducible. The high capacity of this technique to generate DNA markers offers a new possibility for the study of the genetic relationships in the genus Vitis.Abbreviations PCR
Polymerase chain reaction
- RAPD
Random amplified polymorphic DNA |
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| Keywords: | Genetic diversity grapes molecular markers PCR RAPD Vitis |
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