| 早酥梨抗黑星病相关新基因Vnp1的克隆及其表达分析 |
| |
| 引用本文： | 张洪磊,张朝红,王跃进.早酥梨抗黑星病相关新基因Vnp1的克隆及其表达分析[J].农业生物技术学报,2010,18(2):239-245. |
| |
| 作者姓名： | 张洪磊 张朝红 王跃进 |
| |
| 作者单位： | 农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,西北农林科技大学园艺学院,杨凌,712100 |
| |
| 基金项目： | 陕西省重大科技专项计划，国家高技术研究发展计划(863)计划项目 |
| |
| 摘 要： | 在前期已克隆得到的早酥梨(Pyrus bretschneideri cv.Zaosu)抗病基因同源类似物Pb-Zs3(GenBank登录号:EU939783)的基础上,通过RT-PCR获得Pb-Zs3的cDNA序列,并利用RACE技术获得该基因的全长cDNA序列,命名为早酥梨抗黑星病相关基因(Vnp1),提交至GenBank(GenBank登录号:FJ763188).生物信息学分析表明,早酥梨Vnp1基因的全长cDNA序列为1200 bp,包含一个完整的921 bp的开放读码框架(ORF),编码306个氨基酸残基,预测其分子质量为34.6 kD.同时,该基因含有与抗病相关的功能结构域核酸结合位点(NB-ARC),同源性比对显示其与苹果黑星病菌(Venturia inaequails)诱导后的苹果EST序列ABEA004598(GenBank登录号:EB150292)同源性达71%.对早酥梨Vnp1基因进行半定量RT-PCR研究的结果表明,该基因在梨黑星病菌(Venturia nashicola)诱导后216 h内的叶片中的表达水平呈明显上升趋势.
|
| 关 键 词： | 梨 梨黑星病 抗病基因同源类似物 半定量RT-PCR |
Cloning and Expression Analysis of Novel Gene Vnp1 Related to Pear Scab Disease Resistance from Zaosu Pear |
| |
| Zhang Honglei,Zhang Chaohong,Wang Yuejin.Cloning and Expression Analysis of Novel Gene Vnp1 Related to Pear Scab Disease Resistance from Zaosu Pear[J].Journal of Agricultural Biotechnology,2010,18(2):239-245. |
| |
| Authors: | Zhang Honglei Zhang Chaohong Wang Yuejin |
| |
| Institution: | Zhang Honglei Zhang Chaohong Wang Yuejin Key Laboratory of Horticulture Plant Germplasm Utilization,Northwest China of Ministry of Agriculture,Key Laboratory of Agricultural Molecular Biology of Shaanxi Province,College of Horticulture,Northwest Agriculture , Forestry University,Yangling,712100 |
| |
| Abstract: | Based on the disease resistance gene analog Pb-Zs3(GenBank accession No.EU939783) which was cloned from Zaosu pear(Pyrus bretschneideri cv.Zaosu) in previous study, its expressed cDNA sequence was acquired with RT-PCR.The technique of rapid amplification of cDNA end(RACE) was used to clone its full-length cDNA sequence, and this gene was named pear scab related disease resistance gene(Vnp1) which has been submitted to GenBank(GenBank accession No.FJ763188).Bioinformatic Analysis revealed that Vnp1 gene of Z... |
| |
| Keywords: | RACE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
