| 葡萄种质离体保存中低温和矿物油覆盖延长继代间隔的效应 |
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| 引用本文： | 成晓华,徐志微,王莉,师校欣,杜国强.葡萄种质离体保存中低温和矿物油覆盖延长继代间隔的效应[J].园艺学报,2021(3):477-486. |
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| 作者姓名： | 成晓华 徐志微 王莉 师校欣 杜国强 |
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| 作者单位： | 河北农业大学园艺学院;武邑宏达学校 |
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| 基金项目： | 河北省自然科学基金项目(C2018204055);河北省现代农业产业技术体系创新团队建设项目(HBCT2018100204);国家重点研发计划支持项目(2018YFD0201300)。 |
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| 摘 要： | 以'巨峰'等14个葡萄品种试管苗为试材,研究不同保存温度和培养基表面覆盖矿物油对其生长的影响.结果 表明:低温可有效延长试管苗的继代间隔时间,转入常温培养后恢复生长良好,但不同品种适宜的温度不同.设定存活率降至30％～40％时的保存时间为最长继代时间,'红斯威特'莫丽莎'皇家夏天'克瑞森无核'葡萄最适低温9℃,可...
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| 关 键 词： | 葡萄 种质保存 试管苗 延缓生长 继代间隔 低温 预培养 矿物油覆盖 |
Efficacy of Low Temperature and Mineral Oil Covering in Prolonging Subculture Interval During Grape Germplasm Preservation in Vitro |
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| CHENG Xiaohua,XU Zhiwei,WANG Li,SHI Xiaoxin,DU Guoqiang.Efficacy of Low Temperature and Mineral Oil Covering in Prolonging Subculture Interval During Grape Germplasm Preservation in Vitro[J].Acta Horticulturae Sinica,2021(3):477-486. |
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| Authors: | CHENG Xiaohua XU Zhiwei WANG Li SHI Xiaoxin DU Guoqiang |
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| Institution: | (College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China;Wuyi Hongda High School,Hengshui,Hebei 053400,China) |
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| Abstract: | Fourteen grape cultivars were used as plant materials to reveal the influences of low culture temperature and mineral oil covering on medium of plantlets in vitro.The results showed that the low temperature could significantly prolong the subculture period,but the optimal low temperature varied according to the different cultivars.Plantlets cultured in the low temperature could resume growth well after being transferred to routine temperature culture.When the survival rate of 30%–40%was a concern,the optimal temperature was 9℃for’Sweet Scarlet’,’Melissa’,’Summer Royal’and’Crimson Seedless’cultivars,and the subculture period could reach more than 850 days,and maximum 1060 days for certain cultivar;the optimal temperature was 12–15℃for’Kyoho’,’Jumeigui’,’Autumn Royal’,’Flame Seedless’,’Summer Black’,’Princess’,’Lilit’,’Cabernet Sauvignon’,’Manicure Finger’and’Wink’,and the subculture period could last 350 to 600 days.Prior to the low temperature culture,the plantlets needed to be cultured under routine condition for a period of time(preculture).The duration depended on the culture temperature and the varieties,and it was usually 7 to 21 days.By inoculating shoots with single bud and without leaf,as well as adding 6–8 cm mineral oil on the top of the medium,the subculture period could be up to 420 days in routine temperature culture condition while the control was 150 days.The plantlets resumed growth after the mineral oil was removed. |
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| Keywords: | grape germplasm preservation plantlet in vitro retard growth subculture interval low temperature preculture mineral oil covering |
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