| 睾丸肾上腺素能受体和胆碱能受体的分布及神经递质对睾丸间质细胞增殖的影响 |
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| 引用本文： | 霍书英,马爱进,武现军,李玉荣.睾丸肾上腺素能受体和胆碱能受体的分布及神经递质对睾丸间质细胞增殖的影响[J].中国畜牧兽医,2011,38(8):26-30. |
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| 作者姓名： | 霍书英 马爱进 武现军 李玉荣 |
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| 作者单位： | 1. 河北农业大学动物科技学院,河北保定 071001;2. 中国标准化研究院,北京 100088 |
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| 摘 要： | 试验旨在研究不同发育时期小鼠睾丸肾上腺素能受体(β1AR、β2AR、β3AR、α1A、α1B和α1D)和胆碱能受体(M1、M2、M3、M4和M5)mRNA的表达,以及神经递质去甲肾上腺素(norepinephrine,NE)和乙酰胆碱(acetylcholine,Ach)对发育期小鼠睾丸间质细胞增殖的影响。RT-PCR结果表明,β1AR和β2AR mRNA在睾丸发育的3个时期都表达,β3AR、α1A和α1B mRNA在小鼠发育早期的睾丸表达,在成年期睾丸不表达,α1D mRNA在睾丸发育的早期不表达,成年期表达;胆碱能受体M1R、M2R、M3R和M5R mRNA在睾丸发育的3个时期都表达,而M4R mRNA主要在成年期表达。另外通过NE和Ach处理体外培养的发育期睾丸间质细胞,发现Ach处理组BrdU阳性细胞的数量明显增加(P＜0.01)。结果表明,肾上腺素能受体和胆碱能受体在小鼠睾丸的整个发育时期都表达,Ach可促进发育期小鼠睾丸间质细胞的增殖。
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| 关 键 词： | 肾上腺素能受体和胆碱能受体 去甲肾上腺素和乙酰胆碱 睾丸间质细胞 细胞增殖 |
| 收稿时间： | 2011-05-19 |
Expression of Adrenergic and Muscarinic Acetylcholine Receptor in Mouse Testis and Effects of Norepinephrine and Acetylcholine on the Proliferation of Leydig Cells |
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| HUO Shu-ying,MA Ai-jin,WU Xian-jun,LI Yu-rong.Expression of Adrenergic and Muscarinic Acetylcholine Receptor in Mouse Testis and Effects of Norepinephrine and Acetylcholine on the Proliferation of Leydig Cells[J].China Animal Husbandry & Veterinary Medicine,2011,38(8):26-30. |
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| Authors: | HUO Shu-ying MA Ai-jin WU Xian-jun LI Yu-rong |
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| Institution: | 1. College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China;2. China National Institute of Standardization,Beijing 100088,China |
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| Abstract: | The purpose of the present study is to reveal the expression of adrenergic receptors(β1AR,β2AR,β3AR,α1A,α1B and α1D) and cholinergic receptors (M1,M2,M3,M4 and M5) mRNA in mouse testis during different periods,and the effects of NE and Ach on the differentiation of Leydig cells from development mouse testis. RT-PCR results showed that β1AR and β2AR mRNA expressed from postnatal day 1 (PD1) to PD50. β3AR,α1A and α1B mRNA only expressed in the earlier development period,no detected in mature testis. α1D mRNA expressed in mature testis,no detected in the earlier devepoment period. M1R,M2R,M3R and M5R mRNA expressed from newborn to the adult period,however,M4R mRNA only detected in the mature testis. Otherwise, we modified the methods to culture the Leydig cells in vitro on PD15,then treated with NE and Ach. The results showed that Ach improved the numbers of BrdU labeled cells in the culturing Leydig cells(P＜0.01). Adrenergic receptors and cholinergic receptors expressed through all the development stages in mouse testis. Ach enhanced the proliferation of Leydig cells during development stage. |
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| Keywords: | adrenergic receptors and cholinergic receptors norepinephrine (NE) and acetylcholine (Ach) Leydig cells proliferation |
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