| 洼地绵羊FecB基因多态性与其产羔数关系的研究 |
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| 引用本文： | 任艳玲,沈志强,李敏,肖娜,董文艳,李金林,王建军.洼地绵羊FecB基因多态性与其产羔数关系的研究[J].中国畜牧兽医,2011,38(7):159-162. |
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| 作者姓名： | 任艳玲 沈志强 李敏 肖娜 董文艳 李金林 王建军 |
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| 作者单位： | 1. 山东省滨州畜牧兽医研究院,山东滨州 256600;2. 山东省洼地绵羊繁育技术研究推广中心,山东滨州 256600;3. 山东绿都生物科技有限公司,山东滨州 256600 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，山东省自然科学基金，山东省良种工程项目 |
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| 摘 要： | 为了从分子水平上研究洼地绵羊的多羔机制,本研究采用PCR-RFLP方法分析洼地绵羊中FecB基因多态性及其与产羔数的关系。结果表明,洼地绵羊中存在与Booroola羊相同的FecB基因突变,有B/B、B/+、+/+ 3种基因型,其基因型频率分别为0.29、0.56和0.15;且4乳头个体FecB基因频率高于2乳头个体;3种基因型的平均产羔数分别为2.81±0.42、2.49±0.53和1.96±0.28只。经统计分析,B/B与B/+基因型洼地绵羊的产羔数显著高于+/+基因型(P＜0.05),B/B与B/+基因型之间差异不显著(P＞0.05),而+/+基因型个体也存在多羔现象。结果提示,FecB基因可能是洼地绵羊多羔性能主效基因之一,但洼地绵羊可能还存在其它影响多羔性能的基因。
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| 关 键 词： | 洼地绵羊 FecB基因 多态性 产羔数 |
| 收稿时间： | 2010-12-14 |
Association of Polymorphisms in the FecB Gene with Litter Size in Wadi Sheep |
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| REN Yan-ling,SHEN Zhi-qiang,LI Min,XIAO Na,DONG Wen-yan,LI Jin-lin,WANG Jian-jun.Association of Polymorphisms in the FecB Gene with Litter Size in Wadi Sheep[J].China Animal Husbandry & Veterinary Medicine,2011,38(7):159-162. |
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| Authors: | REN Yan-ling SHEN Zhi-qiang LI Min XIAO Na DONG Wen-yan LI Jin-lin WANG Jian-jun |
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| Institution: | 1. Shandong Binzhou Anmimal Science & Veterinary Medicine Institute,Binzhou 256600,China;2. Research and Development Center of Wadi Sheep Breeding Technology,Binzhou 256600,China;3. Shandong Lvdu Biotechnology Ltd., Binzhou 256600,China |
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| Abstract: | To study the fecundity of Wadi sheep on molecular level,polymorphisms within the FecB gene of Wadi sheep and associations with litter size were analyzed by PCR-RFLP. The results showed that there was FecB mutation in Wadi sheep that was the same as in Booroola,and the frequencies of three genotypes (B/B,B/+,+/+) were 0.29,0.56 and 0.15,respectively. An association between genotype frequencies and number of nipples was observed. Average litter sizes for the three genotypes were 2.81±0.42,2.49±0.53 and 1.96±0.28,respectively. Statistical analysis showed that litter size of Wadi sheep with B/B and B/+ genotypes were significantly greater than that of sheep with the +/+ genotypes (P＜0.05),but differences between the B/B and B/+ genotypes were not significant(P＞0.05). These results indicated that the FecB gene could be of great interest in the Wadi sheep breed due to its association with fecundity. |
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| Keywords: | Wadi sheep FecB gene polymorphism litter size |
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