| 草鱼出血病病毒减毒的研究 |
| |
| 引用本文： | 许淑英,李焕林.草鱼出血病病毒减毒的研究[J].上海水产大学学报,1998,7(3):211-216. |
| |
| 作者姓名： | 许淑英 李焕林 |
| |
| 作者单位： | 中国水产科学研究院珠江水产研究所!广州510380 |
| |
| 摘 要： | 草鱼出血病病毒ＧＣＨＶ－８２９野毒株在ＰＳＦ细胞连续传代过程，于培养液中加入一定浓度的桉液，传至１９代已达减毒目的，继续传至２９代其减毒效果保持稳定，２７代后不再加入桉液，继续传至３７代其减毒效果仍然保持稳定不变。致弱病毒对草鱼的安全性好，免疫原性强，用该弱毒株制备的弱毒疫苗免疫草鱼后，草鱼的成活率和免疫保护率均达１００％，表明桉液的草鱼出血病病毒的一种良好减毒剂，经桉液减毒的ＧＣＨＡＶ－８９２是
|
| 关 键 词： | 草鱼 出血病病毒 桉液 减毒 |
STUDY ON ATTENUATION OF VIRUS OF HEMORRHAGE OF GRASS CARP, CTENOPHARYNGODON IDELLA |
| |
| XU Shu-Ying,LI Huan-Lin,DENG Guo-Cheng,JIANG Lan,BAI Yue-Qiang.STUDY ON ATTENUATION OF VIRUS OF HEMORRHAGE OF GRASS CARP, CTENOPHARYNGODON IDELLA[J].Journal of Shanghai Fisheries University,1998,7(3):211-216. |
| |
| Authors: | XU Shu-Ying LI Huan-Lin DENG Guo-Cheng JIANG Lan BAI Yue-Qiang |
| |
| Abstract: | This paper describes that GCHV-892 has been domesticated continuously for 19 generations in cell 1ine (PSF) in culture solution with certain concentration of liquid being from An (Eucalyptus) leaves, and has been attenuated. Effect of attenuation has been stabilized for continuous domestication from 19 to 29 gen. The effect of attenuation has still been stabilized for continuous domestication up to 37 gen. After the An liquid did not add to the culture solution from 27~37 gen., there are good innocuity and strong immunogenicity of virus for grass carp. Attenuated live vaccine prepared with the attenuated strain for grass carp is 100% in innocuity survival rate and immune protection rate. It shows that the An liquid is a good attenuated medicine for virus of hemorrhage of grass carp, and GCHAV-892 attenuated with An liquid is a good attenuated strain. |
| |
| Keywords: | Ctenopharyngodon idella virus of hemorrhage An (Eucalyptus) liquid attenuation |
| 本文献已被 CNKI 维普 等数据库收录！ |
