| 梨抗黑星病AFLP标记筛选 |
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| 引用本文： | 董星光,方成泉,王斐,王志刚,樊丽,林盛华.梨抗黑星病AFLP标记筛选[J].植物病理学报,2010,40(5):552-555. |
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| 作者姓名： | 董星光 方成泉 王斐 王志刚 樊丽 林盛华 |
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| 作者单位： | 中国农业科学院果树研究所, 农业部果树种质资源利用重点开放实验室, 兴城 125100 |
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| 基金项目： | 国家863计划，国家科技支撑计划，中央级公益性科研院所基本科研业务费专项，农业部948项目(2006-G27 |
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| 摘 要： | Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears. Molecular markers linked to scab resistance gene is expected to be useful for improving pear. In this study, the F1 population derived from the cross of ‘Huangguan’ and ‘Yali’ was analyzed genetically. The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner. Bulked segregant analysis (BSA) was conducted to screen 64 fluorescent AFLP primer pairs. A marker designated as D3-365 was found to be linked to the resistant locus. Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
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| 收稿时间： | 2009-10-29 |
Identification of AFLP markers linked to a locus conferring resistance to scab in pear |
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| DONG Xing-guang,FANG Cheng-quan,WANG Fei,WANG Zhi-gang,FAN Li,LIN Sheng-hua.Identification of AFLP markers linked to a locus conferring resistance to scab in pear[J].Acta Phytopathologica Sinica,2010,40(5):552-555. |
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| Authors: | DONG Xing-guang FANG Cheng-quan WANG Fei WANG Zhi-gang FAN Li LIN Sheng-hua |
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| Institution: | Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Key Laboratory of Fruit Germplasm Resources Utilization, Ministry of Agriculture, Xingcheng 125100, China |
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| Abstract: | Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears. Molecular markers linked to scab resistance gene is expected to be useful for improving pear. In this study, the F1 population derived from the cross of ‘Huangguan’ and ‘Yali’ was analyzed genetically. The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner. Bulked segregant analysis (BSA) was conducted to screen 64 fluorescent AFLP primer pairs. A marker designated as D3-365 was found to be linked to the resistant locus. Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM. |
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| Keywords: | pear scab BSA fluorescent AFLP selective genotype analysis |
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