| AB-8大孔树脂分离纯化爵床总黄酮的研究 |
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| 引用本文： | 周燕芳,丁冬纯.AB-8大孔树脂分离纯化爵床总黄酮的研究[J].湖北农业科学,2009,48(12). |
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| 作者姓名： | 周燕芳 丁冬纯 |
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| 作者单位： | 韩山师范学院化学系,广东,潮州,521041 |
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| 摘 要： | 用AB-8大孔吸附树脂对爵床总黄酮进行分离纯化.结果表明,静态分离纯化的最佳工艺参数为提取液中总黄酮量在2.0～3.5 mg/mL范围,AB-8树脂与提取液以1:20(W/V)的比例,于25℃,在酸性或碱性的条件下恒温振荡24h后,用90%的乙醇洗脱.此条件下总黄酮的得率可达38.24%.动态分离纯化的最佳工艺参数为流速1.0mL/min,粗提液中总黄酮量在1.5～2.5 mg/mL范围,在pH值为2的条件下总黄酮的得率为42.72%.
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| 关 键 词： | AB-8大孔树脂 爵床 总黄酮 分离纯化 |
Isolation and Purification of Total Flavonoids from Acanthaceae by AB-8 Resin |
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| ZHOU Yan-fang,DING Dong-chun.Isolation and Purification of Total Flavonoids from Acanthaceae by AB-8 Resin[J].Hubei Agricultural Sciences,2009,48(12). |
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| Authors: | ZHOU Yan-fang DING Dong-chun |
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| Abstract: | The extraction and purification of the flavonoid from acanthaceae was studied by AB-8 resin.The results showed that the effect of dynamic purification of acanthaceae was better than the static purification.The best process parameters for dynandc isolation and pufification were as follows:flow rate Was 1mL/min,flavonoid contents of crude extract were 1.5～2.5mg/mL,pH was 2,the purification yield was 42.72%. |
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| Keywords: | AB-8 resin acanthaceae total flavonoids isolation and purification |
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