| 扩展青霉拮抗菌的筛选鉴定及其发酵液的抑菌效果 |
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| 引用本文： | 刘杨,张海予,韩涛.扩展青霉拮抗菌的筛选鉴定及其发酵液的抑菌效果[J].中国农学通报,2010,26(5):8-13. |
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| 作者姓名： | 刘杨 张海予 韩涛 |
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| 作者单位： | 1. 北京农学院植物科学与技术学院,北京,102206
2. 北京农学院食品系,北京,102206 |
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| 摘 要： | 从水果表面及土壤中分离到几十株细菌，通过平板筛选到6株拮抗效果较好的拮抗菌，其中2株拮抗菌T3和T8的发酵液对扩展青霉（Penicillium expansum）表现出显著的拮抗效果。本实验对该两种菌的发酵液的抑菌作用进行了初步的研究。这两株拮抗菌T3和T8的发酵液稳定性表现出不同，分别用100℃高温或两种蛋白酶处理后，T3菌株的发酵液抑菌效果均有一定程度的下降但仍表现出较高的活性，而T8菌株的发酵液在100℃高温处理后，抑菌活性下降但仍保持部分活性，但用蛋白酶处理，其抗菌活性几乎丧失。T3与T8发酵液的抑菌活性在培养2天后几乎达到最大，T3的最适发酵培养基为BPA，T8的最适发酵培养基为PDA。两株菌的发酵液处理扩展青霉菌的菌丝后，引起菌丝畸形，原生质凝缩，出现空泡。根据细菌16SrDNA序列分析及其形态特征和生理生化特性，初步鉴定T3和 T8均为枯草芽孢杆菌(Bacillus subtilis)，两株菌之间基因序列的相似性为93.45%。
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| 关 键 词： | 砀山酥梨 砀山酥梨 授粉品种 坐果率 脱萼率 |
| 收稿时间： | 2009/10/27 0:00:00 |
| 修稿时间： | 2009/11/13 0:00:00 |
The screening and identification of antagonistic bacteria against Penicillium expansum and the inhibitive effects of fermentation broth |
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| Liu Yang,Zhang Haiyu,HanTao.The screening and identification of antagonistic bacteria against Penicillium expansum and the inhibitive effects of fermentation broth[J].Chinese Agricultural Science Bulletin,2010,26(5):8-13. |
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| Authors: | Liu Yang Zhang Haiyu HanTao |
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| Institution: | Liu Yang1,Zhang Haiyu2,HanTao2 (1Beijing University of Agriculture,Plant science , Technology,Beijing 102206,2Beijing University of Agriculture,Department of Food Science,Beijing 102206) |
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| Abstract: | Penicillium expansum is one of main diseases caused the decay of many fruits. Antagonistic bacteria againstPenicillium expansum are screened from the surface of fruits and the soil. Six strains which have good antagonistic effect are obtained, among them two strains named T3 and T8 which the fermentation broth showed a significant antagonistic effect toPenicillium expansum are found. The antifungal activity of fermentation broth of T3 and T8 are studied. The stability experiments show that after treatment w... |
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| Keywords: | Penicillium expansum antagonistic bacteria inhibitive activity |
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