| 不同光照强度对玫瑰组织培养中初代培养物褐化的影响 |
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| 引用本文： | 杨博,韩振海,张永,李天红.不同光照强度对玫瑰组织培养中初代培养物褐化的影响[J].中国农学通报,2003,19(6):194-194. |
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| 作者姓名： | 杨博 韩振海 张永 李天红 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100094
2. 北京市门头沟区科委,北京,102300 |
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| 基金项目： | 北京市科委资助项目(HO1 0 71 0 1 2 0 1 1 9 |
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| 摘 要： | 玫瑰是一种重要的观赏植物，经济价值高，但玫瑰快繁体系尚未建立。本文研究了在不同田间光照强度下，玫瑰组培初代培养过程中培养物多酚氧化酶（PPO）活性、总酚含量、褐变百分数和萌芽成活率的变化规律。结果表明：遮荫处理使玫瑰培养物的褐变百分数降低而萌芽率显著提高；正常光照强度下多酚氧化酶活性与其他遮荫处理相比并无显著变化，但总酚含量显著高于遮荫处理，二者的相关分析表明多酚氧化酶活性与组织材料的总酚含量相关性不大。
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| 关 键 词： | 玫瑰 光照强度 初代培养 萌芽率 褐变百分数 |
Effect of Different Light Intensity on Browning of Rose (Rosa rugosa) Initially Cultured in vitro |
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| Yang Bo,Han Zhenhai,Zhang Yong,Li Tianhong.Effect of Different Light Intensity on Browning of Rose (Rosa rugosa) Initially Cultured in vitro[J].Chinese Agricultural Science Bulletin,2003,19(6):194-194. |
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| Authors: | Yang Bo Han Zhenhai Zhang Yong Li Tianhong |
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| Institution: | (1College of Agronomy and Biotechnology，China Agricultural University, Beijing 100094；The Committee of Science & Technology of Mentougou District, Beijing 102300) |
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| Abstract: | Rose (Rosa rugosa Thunb ) is an important ornamen tal, but the fast propagation system hasn't been set up yet In this paper, we studied the effect of different light intensity on browning of rose initially cultured in vitro Our results showed that the browning index of rose decreased and the percentage of g ermination increased after field treatment, especially in the light intensity of 5,3331,751 Lx Compared with the light intensity of 5,3331,751 Lx and 63,0781,651 Lx, the activity of PPO of rose under natural light i ntensity showed no significant difference whereas the content of phenolic substa nces was much higher than those treated with shaded illumination The co-effici e ncy analysis demonstrated that there was no obvious relation between the activit y of phenol oxidase and content of phenolic substances |
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| Keywords: | Rose Light intensity Initially Cultured Percenta ge of buds germination Percentage of browning |
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