| 小球藻和亚心扁藻的超低温保存 |
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| 引用本文： | 朱耀强,罗欣夏,宋培学,李洪武.小球藻和亚心扁藻的超低温保存[J].水产养殖,2014(4):42-45. |
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| 作者姓名： | 朱耀强 罗欣夏 宋培学 李洪武 |
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| 作者单位： | [1]海南省海洋与渔业厅,海南海口570228 [2]海南大学海洋学院,海南海口570228 |
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| 摘 要： | 实验采用两步法冷冻技术低温保存小球藻和亚心扁藻,通过冻存后再培养的生长情况的研究,检验两步法冷冻技术低温保存藻种的可行性。实验利用两种不同的抗冻剂对各藻类分别进行预处理、冷冻保存、解冻、再培养,测定相应波段的吸光光度值,以计算叶绿素a的含量,反映各藻类的生长情况。实验结果显示,各藻类经冻存后均有成活,但不同藻在经过不同抗冻剂处理后的生长速度有所不同。
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| 关 键 词： | 抗冻剂 吸光光度值 叶绿素a含量 生长速度 |
The cryopreservation of Chlorella and Platymonas subcordiformis |
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| Zhu Yaoqiang Luo Xinxia Song Peixue Li Hongwu.The cryopreservation of Chlorella and Platymonas subcordiformis[J].Journal of Aquaculture,2014(4):42-45. |
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| Authors: | Zhu Yaoqiang Luo Xinxia Song Peixue Li Hongwu |
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| Institution: | Zhu Yaoqiang Luo Xinxia Song Peixue Li Hongwu (1. Hainan province ocean and fishery hall in Hainan, Haikou 570228 China;2. Hainan university institute of Marine, Haikou 570228, China) |
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| Abstract: | In this research,the technology by two-step freezing to preserve Chlorella and Platymonas subcordiformis at low temperature. And this technology was tested by measuring the concentration of chlorophyll a of these two kinds of algae. This experiment used two kinds of cryoprotectant to pre-handle, cryopreservation, un- freeze and subculture Chlorella and Platymonas subcordiformis. Then we measured the absorption luminosity values so we could know the concentrate of chlorophyll a and the growth rate. The results showed that algae could survive after cryopreservation but the growth speed was various. |
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| Keywords: | cryoprotectant absorption luminosity values concentrate of chlorophyll a growth rate |
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