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Towards the first linkage map of the<Emphasis Type="Italic">Didymella rabiei</Emphasis> genome |
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| Authors: | |
| Institution: | (1) Dept. of Field Crops, Vegetables and Genetics, The Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, 76100 Rehovot, Israel;(2) Dept. of Plant Molecular Biology, Biocenter, University of Frankfurt, D-60439 Frankfurt-am-Main, Germany;(3) Dept. of Plant Pathology, ARO, The Volcani Center, 50250 Bet Dagan, Israel;(4) USDA-ARS, Regional Plant Introduction Station, Washington State University, 99164-6402 Pullman, WA, USA |
| Abstract: | A genetic map was developed for the ascomyceteDidymella rabiei (Kovachevski) v. Arx (anamorph:Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated with 77 F1 progeny derived from crossing an isolate from the U.S.A. and an isolate from Syria. A total of 232 DAF (DNA Amplification Fingerprinting) primers and 37 STMS (Sequence-Tagged Microsatellite Site) primer pairs were tested for polymorphism between the parental isolates; 50 markers were mapped, 36 DAFs and 14 STMSs. These markers cover 261.4cM in ten linkage groups. Nineteen markers remained unlinked. Significant deviation from the expected 1:1 segregation ratios was observed for only two markers (Prob. of χ2<0.05). The implications of our results on ploidy level of the asexual spores are discussed. http://www.phytoparasitica.org posting Sept. 5, 2002. |
| Keywords: | Ascochyta blight chickpea DAF linkage map molecular markers ploidy level STMS |
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