| 芝麻SRAP反应体系的建立与优化 |
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| 引用本文： | 车卓,张艳欣,孙建,黄波,张秀荣.芝麻SRAP反应体系的建立与优化[J].中国农学通报,2008,24(10):74-77. |
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| 作者姓名： | 车卓 张艳欣 孙建 黄波 张秀荣 |
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| 作者单位： | 1. 甘肃农业大学甘肃省作物遗传改良与种质创新重点实验室,兰州,730070;中国农业科学院油料作物研究所,武汉,430062
2. 中国农业科学院油料作物研究所,武汉,430062 |
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| 基金项目： | 国家支撑计划课题，中国农科院油料作物研究所所长基金 |
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| 摘 要： | 以芝麻幼叶提取的DNA为试验材料，通过对影响SRAP扩增结果的重要反应因素dNTPs、Mg2 + 、Taq酶、随机引物及模板DNA进行优化，建立了芝麻扩增多态性高、稳定性强、带型清晰的SRAP最佳反应体系：dNTPs（10mmol／L）0.30μl，Mg2 +（25mmol／L）1.20μl，Taq酶1.00U，正反引物各50ng，DNA模板80ng，10×Buffer 1.5μl，总体积15μl，为SRAP标记技术在芝麻分子生物学研究方面的应用奠定了基础。
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| 关 键 词： | 芝麻 SRAP反应体系 建立与优化 |
| 收稿时间： | 2008-07-30 |
| 修稿时间： | 2008-08-18 |
Establishment and Optimization of SRAP Reaction System in Sesame |
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| Che Zhuo,Zhang Yanxin,Sun Jian,Huang Bo,Zhang Xiurong.Establishment and Optimization of SRAP Reaction System in Sesame[J].Chinese Agricultural Science Bulletin,2008,24(10):74-77. |
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| Authors: | Che Zhuo Zhang Yanxin Sun Jian Huang Bo Zhang Xiurong |
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| Abstract: | The sesame (Sesamum indicum L.) DNA was extracted from young leaf and taken as tested materials. In order to establish the optimum SRAP reaction system which could amplify high levels of polymorphism, good repeatability and clear band pattern, the SRAP amplification system on sesame was optimized from many factors, such as DNA template, Mg2+, dNTPs, Taq DNA polymerase and primers. The optimum SRAP system (total volume of 15μl) was as follows: dNTPs (10mmol／L) 0.30μl, Mg2 + (25mmol／L) 1.20μl, Taq polymerase 1.00U, forward primer 50ng, reverse primer 50ng, DNA template 80 ng, 10×Buffer 1.5μl. This work is a foundation for applying SRAP marker to sesame molecular biology studies. |
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| Keywords: | Sesamezz SRAP reaction systemzz Establishment and optimizationzz |
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