Enzyme treatment for elimination of egg stickiness in tench (Tinca Tinca L.), European catfish (Silurus Glanis L.) and common carp (Cyprinus Carpio L.) |
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| Authors: | O Linhart M Rodina D Gela M Flaj?hans M Kocour |
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| Institution: | 1. Joint Lab. Genet., Physiol. Reprod. Fish, IAPG CAS, University South Bohemia, Research Institute Fish Culture Hydrobiology, 389 25, Vodnany, Czech Republic
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| Abstract: | Enzyme treatment to eliminate egg stickiness in tench, European catfish and common carp was compared with standard methodology in an attempt to decrease time consuming under hatchery conditions. Eggs of tench and E. catfish were exposed to an alcalase enzyme MERCK EC 3.4.21.14 (0.6 AU g?1) solution 3 min after egg fertilization for 2 min. The best enzyme concentration in tench and E. catfish was 10 and 20 ml l?1 of enzyme, respectively. The eggs of c. carp were successfully destickiness with ALCALASE DX (2.5 AU g?1) using two concentration of enzyme (2 ml l?1 and 20 ml l?1) from 8 to 20 min after fertilization. |
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