| 白桃成熟胚试管苗培养研究 |
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| 引用本文： | 汤燕姗,赖钟雄.白桃成熟胚试管苗培养研究[J].江西农业大学学报,2012(4):682-687. |
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| 作者姓名： | 汤燕姗 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所 |
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| 基金项目： | 福建省亚热带果树及特种经济作物种质资源共享平台(2008N2001) |
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| 摘 要： | 以白桃成熟胚为试验材料,无菌萌发获取试管苗,并筛选出试管苗培养最合适的增殖及生根培养基。研究结果表明,白桃试管苗培养最适合的增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.0 mg/L+蔗糖30 g/L+琼脂6 g/L,增殖系数为3.45。生根率最佳诱导培养基为1/4 MS+IBA 0.0 mg/L+NAA 0.4 mg/L+蔗糖30 g/L+琼脂6 g/L,生根率为82.22%。生根数最佳诱导培养基为1/2 MS+IBA 0.5 mg/L+NAA 0.0 mg/L+蔗糖30 g/L+琼脂6 g/L,生根数为5.96。
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| 关 键 词： | 白桃 成熟胚 试管苗 增殖 生根 |
A Study on Culture of the Test-tude Plantlets from Mature Embryo of White Peach |
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| TANG Yan-shan,LAI Zhong-xiong.A Study on Culture of the Test-tude Plantlets from Mature Embryo of White Peach[J].Acta Agriculturae Universitis Jiangxiensis,2012(4):682-687. |
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| Authors: | TANG Yan-shan LAI Zhong-xiong |
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| Institution: | *(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | The test-tube plantlets from mature embryos were used to screen the best proliferation medium and rooting medium.The results showed that the best proliferation medium was MS+6-BA 1.0 mg/L+IBA 0.0 mg/L+sucrose 30 g/L+agar 6 g/L,with the proliferation coefficient of 3.45;the best medium for rooting rate was 1/4 MS +IBA 0.0 mg/L+NAA 0.4 mg/L+sucrose 30 g/L+agar 6 g/L,with the rooting rate of 82.22%;the best medium for rooting number was 1/2 MS +IBA 0.5 mg/L+NAA 0.0 mg/L+ sucrose 30 g/L+agar 6 g/L,with the average root number of 5.96 per plantlet. |
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| Keywords: | white peach mature embryo test-tube plantlet proliferation rooting |
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