| 玉米种胚HSP20基因对人工老化处理的响应 |
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| 引用本文： | 邢芦蔓,吕伟增,雷薇,梁雨欢,卢洋,陈军营.玉米种胚HSP20基因对人工老化处理的响应[J].作物学报,2018,44(11):1733-1742. |
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| 作者姓名： | 邢芦蔓 吕伟增 雷薇 梁雨欢 卢洋 陈军营 |
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| 作者单位： | 河南农业大学农学院, 河南郑州 450046 |
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| 基金项目： | This study was supported by the National Natural Science Foundation of China(31571761) |
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| 摘 要： | 以玉米杂交种“郑单958”为材料, 采用高温(45°C)高湿(100%相对湿度)对玉米种子进行人工老化处理, 并用转录组技术, 研究植物HSP20基因对种子人工老化处理的响应, 旨在为揭示种子衰老的分子机制提供依据。结果表明, 随着老化时间的延长, 种子活力和种胚内过氧化氢酶的活性均表现下降趋势; 过氧化氢含量在老化第3天达到最大值, 随后下降; 丙二醛含量逐渐升高; 转录组检测表明, 种子老化过程中差异显著的HSP20基因有25个, 这些基因编码的HSP20蛋白主要被定位在细胞核、线粒体、以及叶绿体上, 其序列中均含有ACD保守序列(RVDWRETPDAHEIVVDVP GMRREDLRIEVEDNRVLRVSGERRRAEERKGDH WHREERSYGRFWRRFRLPENADLDSVAASLDSGVL TVRFRK)。该序列中含有较多的Arg (11.2%)、Lys (7.2%)、Pro (4.2%)、Thr (3.9%)等氨基酸, 老化过程中积累的ROS可能氧化这些氨基酸, 导致HSP20结构破坏、功能丧失。利用qRT-PCR技术对挑选的编码细胞质、叶绿体和线粒体HSP20的基因的表达模式分析显示, 随着老化程度的加深, 2个编码细胞质HSP20的基因上调表达, 另外两个编码叶绿体和线粒体HSP20基因的表达量在老化第3天达最大值, 随后下降。推测HSP20基因对种子老化有重要作用, HSP20蛋白的ACD结构域中Arg、Lys等氨基酸的靶向氧化可能是种子衰老的主要原因之一。
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| 关 键 词： | 玉米 种胚 种子老化 差异表达基因 HSP20 |
| 收稿时间： | 2018-03-20 |
Response of HSP20 Genes to Artificial Aging Treatment in Maize Embryo |
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| Lu-Man XING,Wei-Zeng LYU,Wei LEI,Yu-Huan LIANG,Yang LU,Jun-Ying CHEN.Response of HSP20 Genes to Artificial Aging Treatment in Maize Embryo[J].Acta Agronomica Sinica,2018,44(11):1733-1742. |
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| Authors: | Lu-Man XING Wei-Zeng LYU Wei LEI Yu-Huan LIANG Yang LU Jun-Ying CHEN |
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| Institution: | College of Agronomy, Henan Agricultural University, Zhengzhou 450046, Henan, China |
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| Abstract: | Maize (Zea mays L.) cultivar ‘Zhengdan 958’ seeds were treated by artificial aging (45°C, 100% relative humidity) and RNA-seq was used to study the response of HSP20 genes to artificial aging treatment of seeds, so as to provide evidences for uncovering the molecular mechanism of seed aging. In the study, the seed vigor decreased dramatically with increasing aging time. The activity of catalase in seed embryo showed a decreasing trend. The content of hydrogen peroxide increased to the maximum at the third day of aging and then decreased. The content of malonaldehyde increased, which indicated that the damage to the membrane system increased. Twenty-five HSP20 genes were identified. These genes encoded HSP20 and mainly distributed in nuclei, mitochondria and chloroplasts. There was a conserved ACD sequence (RVDWRETPDAHEIVVDVPGMRREDLRIEVE DNRVLRVSGERRRAEERKGDHWHREERSYGRFW RRFRLPENADLDSVAASLDSGVLTVRFRK) in HSP20 protein which contains more amino acids, such as Arg (11.2%), Lys (7.2%), Pro (4.2%), and Thr (3.9%). In the aging process these amino acids might be oxidized by ROS accumulated in the embryo, leading to protein structure damaged and loss of functions. The expression patterns of HSP20 genes in cytoplasm, chloroplasts and mitochondria were validated by qRT-PCR, showing that the expressions of cytoplasmic HSP20 genes were up-regulated and those of chloroplast and mitochondrial HSP20 genes reached peak at the third day of aging treatment, and then declined. These suggested that the HSP20 genes play an important role in seeds aging, and the targeted oxidation of Arg, Lys and other amino acids in the ACD structural domain may be an essential cause leading to seed deterioration. |
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| Keywords: | maize seed embryo seed aging differential expressed genes HSP20 |
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