| 葡萄5BB品种SRAP-PCR反应体系影响因素 |
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| 引用本文： | 金美玉,修景润,廉美兰,朴炫春.葡萄5BB品种SRAP-PCR反应体系影响因素[J].中国农学通报,2010,26(20):33-37. |
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| 作者姓名： | 金美玉 修景润 廉美兰 朴炫春 |
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| 作者单位： | 1. 延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉,133002
2. 延边农业科学院,吉林龙井,133400 |
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| 基金项目： | 延边大学科技发展计划项目(延大科合字 |
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| 摘 要： | 为建立适合葡萄5BB品种的SRAP-PCR反应体系,利用正交设计对葡萄SRAP-PCR反应体系5种因素(Taq DNA聚合酶,Mg2+,模板DNA,dNTP,引物)4个水平进行优化。结果表明,各因素水平变化对PCR反应的影响从大到小顺序为:Mg2+,引物,dNTP,Taq DNA聚合酶,模板DNA;筛选出各因素的最佳水平,建立了葡萄5BB品种SRAP-PCR反应的最佳体系(20μL)为:Taq DNA聚合酶2U,Mg2+2.0mmol/L,模板DNA60ng,dNTP0.25mmol/L,引物0.10μmol/L。这一优化系统的建立为今后利用SRAP标记技术对葡萄进行相关研究提供了帮助。
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| 关 键 词： | 水产养殖 水产养殖 循环水养殖系统 pH值 碱性试剂 |
| 收稿时间： | 7/9/2010 12:00:00 AM |
| 修稿时间： | 2010/7/30 0:00:00 |
Influence Factors of the SRAP-PCR Reaction System on Grape 5BB |
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| Jin Meiyu,Xiu Jingrun,Lian Meilan,Piao Xuanchun.Influence Factors of the SRAP-PCR Reaction System on Grape 5BB[J].Chinese Agricultural Science Bulletin,2010,26(20):33-37. |
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| Authors: | Jin Meiyu Xiu Jingrun Lian Meilan Piao Xuanchun |
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| Institution: | 1 (1Key Laboratory of Organism Functional Factors of the Changbai Mountain of Yanbian University,Ministry of Education,Yanji Jinlin 133002; 2YanBian Academy of Agricultural Sciences,Longjing Jilin 133400) |
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| Abstract: | To established a SRAP-PCR reaction system for the grape 5BB. In this paper,the orthogonal design was used to optimize SRAP-PCR amplification system on grape on four levels of five factors （Taq DNA polymerase,Mg2＋,DNA template,dNTPs,and primer,respectively）. The results showed the order of each factor in different levels affected on the result of PCR was Mg2 ＋ ,primer,dNTPs,Taq DNA polymerase and DNA template. A most suitable SRAP-PCR system for grape 5BB was established that was total 20 μL reaction system containing 2 UTaq DNA polymerase,2.0 mmol/L Mg2＋,60 ng DNA template,0.25 mmol/L dNTPs,and 0.10 μmol/L primer. This optimized system for SRAP marker would become one of the protocols for further research on grape. |
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| Keywords: | SRAP |
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