| sbk和sck双价抗虫转基因水稻的育成和鉴定 |
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| 引用本文： | 李聪,张启军,刘少奎,赖东,漆庆明,吕川根.sbk和sck双价抗虫转基因水稻的育成和鉴定[J].中国水稻科学,2011,25(6):599-604. |
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| 作者姓名： | 李聪 张启军 刘少奎 赖东 漆庆明 吕川根 |
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| 作者单位： | 南京农业大学农学院;江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心; |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2008348); 国家转基因生物新品种培育科技重大专项(2008ZX08001-004) |
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| 摘 要： | 将包含2个抗虫基因sbk\修饰后的Cry1A(c)\]和sck（修饰后的CpTI）的质粒载体pCDMARUBA Hyg转入农杆菌EHA105中, 感染南粳45的愈伤组织, 得到再生植株。用sbk和sck基因的引物进行PCR分析, 从97个再生植株中筛选出42个含有2个抗虫基因而没有潮霉素基因的阳性植株。通过Southern杂交发现, 42个阳性植株中具有1~5个外源基因拷贝的分别有23、 11、 5、2和1个单株。用RT PCR检测发现, 4个单拷贝株衍生的28个T3代植株外源基因能正常表达。人工饲喂二化螟幼虫试验也表明, 转基因植株的幼虫死亡率达94%~100%, 抗虫能力比对照显著提高。筛选出3个农艺性状优良的株系。
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| 关 键 词： | 水稻 sbk基因 sck基因 抗虫 转基因 育种 |
| 收稿时间： | 2010-12-08; |
Breeding and Identification of Insect-Resistant Rice with Two Insecticidal Genes,sbk and sck |
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| LI Cong,#,ZHANG Qi-jun,LIU Shao-kui,LAI Dong,QI Qing-ming,LU Chuan-gen.Breeding and Identification of Insect-Resistant Rice with Two Insecticidal Genes,sbk and sck[J].Chinese Journal of Rice Science,2011,25(6):599-604. |
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| Authors: | LI Cong # ZHANG Qi-jun LIU Shao-kui LAI Dong QI Qing-ming LU Chuan-gen |
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| Institution: | LI Cong1,#,ZHANG Qi-jun2,LIU Shao-kui1,LAI Dong1,QI Qing-ming2,LU Chuan-gen2,(1College of Agronomy,Nanjing Agricultural University,Nanjing 210095,China,2Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center,Nanjing 210014,# These authors contributed equally to this paper) |
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| Abstract: | The plasmid of pCDMARUBA-Hyg,which contained two insect-resistant genes,sbkmodified from Cry1A(c)] and sck(modified from CpTI),was transformed into an Agrobacterium EHA105 for infection of the calli of a super japonica rice Nanjing 45.Plants regenerated from the transformation were subjected to all kinds of gene verifications and selection.Using PCR detection with the primers of sbk and sck genes,42 positive transgenic plants that were marker-free and contained the two target genes were selected from 97 re... |
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| Keywords: | Oryza sativa sbk gene sck gene insect resistance transgene breeding |
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