| 抑制水稻隐花色素基因OsCRY1a表达对水稻农艺性状的影响 |
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| 引用本文： | 李毓,庄伟建,王乃元,洪国琴,戴飞.抑制水稻隐花色素基因OsCRY1a表达对水稻农艺性状的影响[J].中国水稻科学,2011,25(6):575-579. |
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| 作者姓名： | 李毓 庄伟建 王乃元 洪国琴 戴飞 |
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| 作者单位： | 福建农林大学作物科学学院;福建农林大学作物遗传育种与综合利用教育部重点实验室;福建省分子与细胞生物学重点实验室; |
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| 基金项目： | 福建省科技计划重点资助项目(2008I01021117); 国家863计划资助项目(2007AA10Z181) |
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| 摘 要： | 利用已公布的OsCRY1a基因的序列设计引物，扩增部分基因片段，构建RNAi植物表达载体并转化水稻，导致该基因表达水平下降和功能缺失。根据转基因植株重要农艺性状的表现，分析该基因的功能。结果表明，抑制OsCRY1a基因的表达，水稻开花期推迟16 d，株高和粒长明显增加，而其他重要农艺性状如剑叶长、宽和穗长等无明显变化。
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| 关 键 词： | 水稻 隐花色素基因 OsCRY1a 农艺性状 基因表达 |
| 收稿时间： | 2011-01-03; |
Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits |
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| LI Yu,ZHUANG Wei-jian,WANG Nai-yuan,HONG Guo-qin,DAI Fei.Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits[J].Chinese Journal of Rice Science,2011,25(6):575-579. |
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| Authors: | LI Yu ZHUANG Wei-jian WANG Nai-yuan HONG Guo-qin DAI Fei |
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| Institution: | LI Yu1,2,ZHUANG Wei-jian1,3,WANG Nai-yuan2,HONG Guo-qin1,DAI Fei3 (1 College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China,2 Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,3Key Laboratory of Molecular and Cellular Biology,China) |
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| Abstract: | Using primers designed according to the published sequence of OsCRY1a gene,part of the gene fragment was obtained by PCR and a RNAi expression vector was constructed.The vector was then introduced into rice,leading to the downregulation of the expression level and loss-of-function of the gene.Based on the performance of the transgenic plants,the functions of OsCRY1a was analyzed and deduced.The results indicated that suppressing the expression of OsCRY1a retarded flowering for 16 d with the plant height and... |
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| Keywords: | rice cryptochrome gene OsCRY1a agronomic trait gene expression |
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