|
|||||
|
|
| 葡萄组培苗日光温室微型扦插扩繁技术 | |
| 引用本文： | 郭恩才,谭鑫,李海山.葡萄组培苗日光温室微型扦插扩繁技术[J].河北农业科学,2010,14(3):8-9,33. |
| 作者姓名： | 郭恩才 谭鑫 李海山 |
| 作者单位： | 1. 河北省农林科学院昌黎果树研究所,河北,昌黎,066600 2. 石家庄市科技信息研究所,河北,石家庄,050000 3. 河北省农林科学院石家庄果树研究所,河北,石家庄,050061 |
| 摘 要： | 利用日光温室进行葡萄试管苗微型茎段扦插的扩繁方法,是生物技术领域的1项创新,取代了传统的组培培养室继代生产扩繁模式,实现了常规条件下的扩繁生产。简述了葡萄试管苗日光温室微型扦插扩繁技术,并将利用该技术扩繁的小苗与传统培养的小苗在生长特性及抗病性方面进行了比较,还比较了该技术与传统技术的扩繁效率。结果表明:该技术提高了扩繁增殖效率2~3倍,大大降低了生产成本;生产扩繁的幼苗健壮,株型紧凑,叶片浓绿,抗病性强,外移成活率高。为今后生物技术领域大规模组培扩繁、生产开发,探索了1条实用化较强的技术途径。 |
| 关 键 词： | 葡萄 日光温室 试管苗 微型扦插 扩繁 |
Micro-cutting Propagation Technology of Grape Plantlet in Vitro in Solar Greenhouse |
|
| GUO En-cai,TAN Xin,LI Hai-shan.Micro-cutting Propagation Technology of Grape Plantlet in Vitro in Solar Greenhouse[J].Journal of Hebei Agricultural Sciences,2010,14(3):8-9,33. | |
| Authors: | GUO En-cai TAN Xin LI Hai-shan |
| Institution: | 1.Changli Institute of Pomology,Hebei Academy of Agriculture and Forestry Sciences,Changli 066600,China;2.Shijiazhuang Institute of Sci-tech Information,Shijiazhuang 050000,China;3.Shijiazhuang Institute of Pomology,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050061,China) |
| Abstract: | The micro-cutting propagation technology of grape plantlet in vitro in solar greenhouse was an innovation in biotechnology field,which replaced the traditional subculture propagation in tissue culture works,and realized the propagation under conventional conditions.The micro-cutting propagation technology of grape plantlet in vitro in solar greenhouse was briefly introduced.The growth characteristics and diseases resistances of plantlet in vitro and propagation efficiency of micro-cutting propagation technology and traditional technology were compared.The results showed that this new method reduced greatly production cost,improved propagation efficiency and had much virtue,such as stronger seedling,compact plant type,dark green leaves,strong disease resistance and high transplanting survival rate.This technique provided a practical approaches to the big scale clone propagation and production development in biotechnology. |
| Keywords: | Grape Solar greenhouse Plantlet in vitro Micro-cutting Propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |