| Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea |
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| 作者姓名： | ZHENG Wu CHEN Ji-sheng ZHENG Shi-qin LU Guo-dong WANG Zong-hua |
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| 作者单位： | Key Laboratory of Bio-pesticide and Chemistry Biology, Ministry of Education/Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China |
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| 摘 要： | MgCdc42 （Cdc42 in Magnaporthe grisea）, with high homology to ScCdc42 （Cdc42 in Saccharomyces cerevisiae）, has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network, the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBeml, Chin1, and MgGicl in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRgal, and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.
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| 关 键 词： | 鸟嘌呤 蛋白质 同源性 细胞分裂 作物生理 |
Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea |
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| ZHENG Wu CHEN Ji-sheng ZHENG Shi-qin LU Guo-dong WANG Zong-hua.Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea[J].Agricultural Sciences in China,2006,5(10):780-786. |
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| Authors: | ZHENG Wu CHEN Ji-sheng ZHENG Shi-qin LU Guo-dong WANG Zong-hua |
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| Institution: | Key Laboratory of Bio-pesticide and Chemistry Biology, Ministry of Education/Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China |
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| Abstract: | MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network, the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBeml, Chml, and MgGicl in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRgal, and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway. |
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| Keywords: | Cdc42 Magnaporthe grisea guanine nucleotide exchange factor GTPase-activating protein |
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