| 修饰的ORF5基因增强猪繁殖与呼吸综合征DNA疫苗的体液免疫 |
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| 引用本文： | 江云波,方六荣,肖少波,牛传双,张辉,陈焕春.修饰的ORF5基因增强猪繁殖与呼吸综合征DNA疫苗的体液免疫[J].中国兽医学报,2005,25(1):1-3. |
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| 作者姓名： | 江云波 方六荣 肖少波 牛传双 张辉 陈焕春 |
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| 作者单位： | 华中农业大学,动物医学院,湖北,武汉,430070 |
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| 基金项目： | 武汉市青年科技晨光计划资助项目 (2 0 0 2 5 0 0 10 41) |
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| 摘 要： | 为了提高猪繁殖与呼吸综合征病毒(PRRSV)ORF5基因DNA疫苗的免疫效力，将通用型辅助性T淋巴细胞表位(PADRE)插入ORF5的中和表位和覆盖表位间，获得修饰后的ORF5基因ORF5M。在此基础上，进一步构建了ORF5M的真核表达质粒pCl-52M。间接免疫荧光证实体外表达后，免疫BALB／c小鼠，检测免疫后的ELISA抗体和中和抗体，并与未经修饰的ORF5基因真核表达质粒pCl-52进行比较。结果表明，修饰后的DNA疫苗pCl-52M诱导的ELISA抗体和中和抗体均明显优于未经修饰的DNA疫苗pCl-52，是一种具有良好开发前景的PRRS新型疫苗。
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| 关 键 词： | ORF5基因 ELISA抗体 猪繁殖与呼吸综合征病毒 PRRSV 新型疫苗 免疫效力 覆盖 DNA疫苗 中和抗体 体液免疫 |
| 文章编号： | 1005-4545(2005)01-0001-03 |
| 修稿时间： | 2003年9月21日 |
Enhanced Humoral Immune Response of DNA Vaccine Composing a Modified ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus |
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| JIANG Yun-bo,FANG Liu-rong,XIAO Shao-bo,NIU Chuan-shuang,ZHANG Hui,CHEN Huan-chun.Enhanced Humoral Immune Response of DNA Vaccine Composing a Modified ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus[J].Chinese Journal of Veterinary Science,2005,25(1):1-3. |
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| Authors: | JIANG Yun-bo FANG Liu-rong XIAO Shao-bo NIU Chuan-shuang ZHANG Hui CHEN Huan-chun |
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| Institution: | JIANG Yun-bo,FANG Liu-rong,XIAO Shao-bo,NIU Chuan-shuang,ZHANG Hui,CHEN Huan-chun~* |
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| Abstract: | To enhance the immunogenicity of DNA vaccines encoding the GP5 of porcine reproductive and respiratory syndrome virus(PRRSV),the universal helper T-cell antigenic epitope(PADRE) was introduced into the neutralization epitope and the decoy epitope of the ORF5 gene,resulting in a modified ORF5 gene(ORF5M).The modified ORF5M was further cloned into the eukaryotic expression vector pCI-neo and the expression was confirmed by indirect immunofluorescence assay.BALB/c mice were immunized with either the modified expression plasmid pCI-52M or non-modified expression plasmid pCI-52 and the humoral immune responses were elevated at various time points after primary immunization.Results showed that both ELISA and neutralization antibody titers induced by the modified DNA vaccine pCI-52M were higher significantly than those elicited by non-modified DNA vaccine pCI-52,indicating that the modified DNA vaccine pCI-5M may enhance the potency of PRRSV DNA vaccines and can be considered as a promising vaccine against PRRS. |
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| Keywords: | PRRSV ORF5 gene PADRE epitope DNA vaccines |
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