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| 应用ISSR分析油茶无性系的遗传多样性 | |
| 引用本文： | 王保明,陈永忠,谭晓风,彭邵锋,石明旺.应用ISSR分析油茶无性系的遗传多样性[J].东北林业大学学报,2008,36(6):19-24. |
| 作者姓名： | 王保明 陈永忠 谭晓风 彭邵锋 石明旺 |
| 作者单位： | 1. 经济林育种与栽培国家林业局重点实验室(中南林业科技大学),长沙,410004 2. 湖南省林业科学院 3. 中南林业科技大学 |
| 基金项目： | 林业部科研项目 , 科技部农业科技成果转化基金 , 国家科技支撑计划 |
| 摘 要： | 以23个油茶无性系叶片的DNA作为实验材料,从60个随机引物中筛选出10个最优引物,并扩增出102条谱带,其中多态性谱带70条,多态性比率为68.6%,谱带大小为200～2000bp。Shannon遗传信息指数为0.4793,平均观察等位基因数和有效等位基因数分别为2.000和1.519;遗传距离为0.1542～0.6931,油茶无性系之间具有较高多态性。运用平均聚类法分析23个油茶无性系的亲缘关系及多样性,在遗传距离为0.392时,23个油茶无性系聚为4类:湘林156、湘林210和湘林4号等18个油茶无性系为第1类;岑软2号和湘林97号为第2类;湘林34号和湘林11为第3类;湘林81号单独为1类。其中,湘林4号与湘林29的亲缘关系最近,湘林34与湘林78的亲缘关系最远。在此基础上,分析了油茶ISSR聚类结果与油茶农艺学性状的关系,从而为油茶优良无性系甄别、亲缘关系分析以及种质资源利用,在分子水平上积累了有效证据。 |
| 关 键 词： | ISSR 油茶 优良无性系 遗传多样性 |
| 修稿时间： | 2007年7月20日 |
Genetic Diversity of Elite Clones of Camellia oleifera by ISSR |
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| Wang Baoming,Chen Yongzhong,Tan Xiaofeng,Peng Shaofeng,Shi Mingwang.Genetic Diversity of Elite Clones of Camellia oleifera by ISSR[J].Journal of Northeast Forestry University,2008,36(6):19-24. | |
| Authors: | Wang Baoming Chen Yongzhong Tan Xiaofeng Peng Shaofeng Shi Mingwang |
| Abstract: | The genomic DNA extracted from tender leaves of 23 elite clones of Camellia oleifera(Native species for edible oil in China)was used to perform ISSR(inter-simple sequence repeat)analysis.Of the 60 primers screened,10 pieces of DNA fingerprint profiles containing 102 bands with 70 distinct polymorphic bands(68.6%)were obtained,and each primer produced 3 to 10 bands with the band size ranging from 200 bp to 2 000 bp.Result indicated that Shannon's information index was 0.479 3,and the mean observed number of alleles and the mean effective number of alleles were 2.000 0 and 1.518 5,respectively.The genetic distance varied from 0.154 2 to 0.693 1.There exists abundant polymorphism among these elite clones of C.oleifera.The UPGMA analysis result shows that the 23 elite clones of C.oleifera could be classified into 4 groups,namely,18 elite clones(including Xianglin 156,Xianglin 210,Xianglin 4,etc)belong to the first group,Jinruan 2 and Xianglin 97 to the second group,Xianglin 34 and Xianglin 11 to the third group,Xianglin 81 to the fourth group when the genetic distance is 0.392.On the basis of these analyses,the relationship between the clustering result and the agricultural characters of C.oleifera was discussed,which provides some references for the identification of elite clones,phylogenetic analyses and the utilization of germplasm resources. |
| Keywords: | Inter-simple sequence repeat Camellia oleifera Elite clones Genetic diversities |
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