| 羌活基因组DNA提取方法 |
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| 引用本文： | 周鹤峰,张桂彬,邵敏,唐历波,李官成.羌活基因组DNA提取方法[J].安徽农业科学,2008,36(10):3993-3994. |
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| 作者姓名： | 周鹤峰 张桂彬 邵敏 唐历波 李官成 |
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| 作者单位： | 遵义医学院珠海校区生物工程系,广东珠海,519041;高密市第二中学生物教研室,山东高密,261500 |
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| 摘 要： | 目的]探索一种用于RAPD分析的羌活基因组DNA提取方法。方法]分别采取CTAB法S、DS法和改良的CTAB法提取羌活基因组DNA,通过紫外分光光度法、琼脂糖凝胶电泳和RAPD分析对提取的DNA进行检测。结果]3种方法均能有效地从羌活中获得较高产量的DNA,以改良的CTAB法所得的DNA纯度最高,此法提取的羌活基因组DNAOD260/OD280为1.8~2.0,DNA干重得率约为0.235μg/mg。结论]改良的CTAB法更适于羌活基因组DNA的提取,可以完全满足RAPD扩增的需要。
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| 关 键 词： | 羌活 DNA提取 CTAB法 RAPD |
| 文章编号： | 0517-6611(2008)10-03993-02 |
| 修稿时间： | 2007年12月10 |
Study on Methods for Extration of Genomic DNA from Rhizcma et Radix Notopterygii |
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| ZHOU He-feng.Study on Methods for Extration of Genomic DNA from Rhizcma et Radix Notopterygii[J].Journal of Anhui Agricultural Sciences,2008,36(10):3993-3994. |
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| Authors: | ZHOU He-feng |
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| Abstract: | Objective]To explore a method of extracting genomic DNA from Rhizoma et Radix Notopterygii,which could be used in RAPD analysis.Methods]The genomic DNA was extracted from Rhizoma et Radix Notopterygii by SDS,CTAB and modified CTAB method.The genomic DNA obtained by the methods above were tested by ultraviolet spectrophotometry and RAPD.Result]The results showed that the high-yield of DNA can be obtained with all the methods.The purity of DNA extracted with modified CTAB method was the best,the OD260/OD280 ratio of the genomic DNA extracted with modified CTAB method was between 1.8 and 2.0 and the DNA yield was about 0.235 μg/mg dry weight.Conclusion]The modified CTAB method was suitable for the extraction of the genomic DNA from Rhizoma et Radix Notopterygii,the quality of the genomic DNA was high and could be used for RAPD analysis. |
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| Keywords: | Rhizoma et Radix Notopterygii DNA extraction CTAB method RAPD |
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