| 重组死亡素-蜂毒素杂合肽表达·分离纯化的研究 |
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| 引用本文： | 张莉,姚婷,葛定斌,徐小俊,沈宇英,马燕,黄俊杰,钱坤.重组死亡素-蜂毒素杂合肽表达·分离纯化的研究[J].安徽农业科学,2010,38(28):15513-15514. |
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| 作者姓名： | 张莉 姚婷 葛定斌 徐小俊 沈宇英 马燕 黄俊杰 钱坤 |
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| 作者单位： | 湖州师范学院生命科学学院,浙江湖州,313000;浙江省湖州市质量监督检验所,浙江湖州,313000 |
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| 基金项目： | 浙江省新苗人才计划项目，浙江省教育厅项目 |
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| 摘 要： | 目的]采用pET-28a系统包涵体表达死亡素-蜂毒素杂合肽。方法]将死亡素基因重组到pET-28a质粒上,转化到大肠杆菌BL21中构建基因工程菌,SDS-PAGE电泳检测工程菌诱导表达时间,亲和层析纯化目的蛋白。结果]表达的目的蛋白为包涵体,工程菌诱导表达时间8h最佳。结论]包涵体方式表达死亡素-蜂毒素可以获得较高的表达量,纯化后蛋白纯度较高。
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| 关 键 词： | 死亡素-蜂毒素 包涵体 表达 纯化 |
Expression and Purification of Thanatin-Melittin Hybrid Peptide |
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| Institution: | ZHANG Li et al (School of Life Science, Huzhou Teachers＇ College, Huzhou, Zhejiang 313000) |
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| Abstract: | Objective]The research aimed to express thanatin-melittin hybrid peptide with pET-28a expression system.Method]Recombinant plasmid inserted thanatin-melittin gene was transformed into E.coli BL21,and culturing time of engineering bacterial after inducing was studied by SDS-PAGE electrophoretic analysis.Recombinant protein was purified by affinity chromatography.Result] Recombinant protein was in inclusion body,and ideal inducing time was 8 hours.Conclusion]High expression and purification of thanatin-melittin hybrid peptide could be gotten. |
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| Keywords: | Thanatin-Melittin Inclusion body Expression Purification |
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