| Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10. 1 Ecoding Gene from Xinjiang Wheat （Triticum petropavlovskyi Udacz. et Migusch） |
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| 作者姓名： | JIANG Qian-tao WEI Yu-ming WANG Ji-rui YAN Ze-hong ZHENG You-liang |
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| 作者单位： | [1]Triticeae Research Institute, Sichuan Agricultural University, Ya an 625014, P.R.China [2]Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya an 625014,P. R. China |
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| 摘 要： | A novel HMW glutenin subunit gene 1Dy10. 1 was isolated and characterized from Xinjiang wheat （Triticum petropavlovskyi. Udacz. et Migusch） accession Daomai 2. The complete open reading frame （ORF） of 1Dy10. 1 was 1 965 bp, encoding 655 amino acids. The numbers and distribution of cysteines in 1Dy 10.1 were similar to those of 1Dy10 and other y-type subunits. In the N-terminal of 1Dy 10.1, an amino acid was changed from L （leucine） to P （proline） at position 55. The repetitive domain of 1Dy10.1 differed from those of known HMW subunits by substitutions, insertions or/and deletions involving single or more amino acid residues. In the repetitive domain of subunit 1Dyl 0.1, the deletion of tripeptide GQQ in the consensus unit PGQGQQ resulted in the appearance of the motif PGQ that have not been observed in other known y-type HMW subunits. In comparison with the subunit 1Dy 12, a deletion of dipeptide GQ, which occurred in subunit IDy10, was also observed in subunit 1Dy10.1. The cloned IDylO. 1 gene had been successfully expressed in Escherichia coli, and the expressed protein had the identical mobility with the endogenous subunit IDy10.1 from seed.
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| 关 键 词： | 麦谷蛋白 HMW-GS基因 细菌表达 小麦 序列分析 新疆 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-12-23 |
Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10.1 Ecoding Gene from Xinjiang Wheat (Triticum petropavlovskyi Udacz. et Migusch) |
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| JIANG Qian-tao WEI Yu-ming WANG Ji-rui YAN Ze-hong ZHENG You-liang.Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10.1 Ecoding Gene from Xinjiang Wheat (Triticum petropavlovskyi Udacz. et Migusch)[J].Agricultural Sciences in China,2006,5(2):81-89. |
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| Authors: | Qian-tao JIANG Yu-ming WEI Ji-rui WANG Ze-hong YAN You-liang ZHENG |
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| Institution: | aTriticeae Research Institute, Sichuan Agricultural University, Ya'an 625014, P.R. China;bKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education/Sichuan Agricultural University, Ya'an 625014, P.R. China |
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| Abstract: | A novel HMW glutenin subunit gene 1Dy10.1 was isolated and characterized from Xinjiang wheat (Triticum petropavlovskyi. Udacz. et Migusch) accession Daomai 2. The complete open reading frame (ORF) of 1Dy10.1 was 1965 bp, encoding 655 amino acids. The numbers and distribution of cysteines in 1Dy10.1 were similar to those of 1Dy10 and other y-type subunits. In the N-terminal of 1Dy10.1, an amino acid was changed from L (leucine) to P (proline) at position 55. The repetitive domain of 1Dy10.1 differed from those of known HMW subunits by substitutions, insertions or/and deletions involving single or more amino acid residues. In the repetitive domain of subunit 1Dy10.1, the deletion of tripeptide GQQ in the consensus unit PGQGQQ resulted in the appearance of the motif PGQ that have not been observed in other known y-type HMW subunits. In comparison with the subunit 1Dy12, a deletion of dipeptide GQ, which occurred in subunit 1Dy10, was also observed in subunit 1Dy10.1. The cloned 1Dy10.1 gene had been successfully expressed in Escherichia coli, and the expressed protein had the identical mobility with the endogenous subunit 1Dy10.1 from seed. |
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| Keywords: | Triticum petropavlovskyi sequence analysis HMW-GS gene bacterial expression |
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