| 冬小麦miR398前体的克隆及其在低温条件下对靶基因CSD1表达的调控 |
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| 引用本文： | 梅 琳,徐庆华,苍 晶,卢秋巍,于 晶,张 达,冯明芳,张舒驰,梁桂花,张 超.冬小麦miR398前体的克隆及其在低温条件下对靶基因CSD1表达的调控[J].麦类作物学报,2016,36(11):1419-1425. |
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| 作者姓名： | 梅 琳 徐庆华 苍 晶 卢秋巍 于 晶 张 达 冯明芳 张舒驰 梁桂花 张 超 |
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| 作者单位： | 东北农业大学生命科学学院植物生理与分子生物学研究室,黑龙江哈尔滨,150030 |
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| 基金项目： | 黑龙江省自然科学基金项目(C201418)；东北农业大学科学研究基金项目(2012RCB102)；国家自然科学基金项目(31471423)；国家自然科学基金人才培养科研训练项目(J1210069)；国家自然科学基金人才培养条件建设项目(J1120002)；黑龙江省教育厅科学技术研究项目(12541002) |
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| 摘 要： | miR398是受逆境胁迫负调控的miRNA,其靶基因CSD编码超氧化物歧化酶(SOD),使植物抵御活性氧(ROS)的毒害。为进一步了解低温胁迫下miR398的调控机制,从东农冬麦1号中克隆小麦miR398前体,构建过表达载体并转化拟南芥,用Real-time PCR检测T0代植株中小麦miR398及其靶基因CSD1在低温胁迫下的表达量。结果表明,随着低温胁迫时间的延长,miR398表达下调、CSD1基因表达上调,认为小麦miR398能响应低温胁迫、负调控CSD1基因表达,间接提高了拟南芥的抗寒性。
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| 关 键 词： | 冬小麦 miR398 CSD1 抗寒性 表达调控 |
Cloning of Winter Wheat Pre- miR398 and Expression Analysis of Target Gene CSD1 under Cold Stress |
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| MEI Lin,XU Qinghu,CANG Jing,LU Qiuwei,YU Jing,ZHANG D,FENG Mingfang,ZHANG Shuchi,LIANG Guihu,ZHANG Chao.Cloning of Winter Wheat Pre- miR398 and Expression Analysis of Target Gene CSD1 under Cold Stress[J].Journal of Triticeae Crops,2016,36(11):1419-1425. |
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| Authors: | MEI Lin XU Qinghu CANG Jing LU Qiuwei YU Jing ZHANG D FENG Mingfang ZHANG Shuchi LIANG Guihu ZHANG Chao |
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| Abstract: | miR398 is a miRNA that is negatively regulated by the adversity. The target gene CSD encoding superoxide dismutase (SOD) can resist the toxicity of ROS in plants. In order to further understand the regulatory mechanism of miR398 under low temperature stress,the wheat miR398 precursor gene was cloned from Dongnongdongmai 1,and overexpression vector was conducted and transformed into Arabidopsis thaliana,and Real-time PCR was used to detect the expression level of miR398 and its target gene CSD1 in T0 generation under cold stress.The results showed that miR398 of wheat responded to cold stress and down-regulated the expression of CSD1 in Arabidopsis.With the extension of low temperature stress time,the expression of miR398 was down-regulated and the expression of CSD1 was up-regulated. It was suggested that the wheat miR398 could respond to low temperature stress and negatively regulate CSD1 gene expression,which indirectly enhanced the cold resistance of Arabidopsis thaliana. |
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| Keywords: | Winter wheat miR398 CSD1 Cold resistance Gene expression regulation |
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