| 桂花幼胚的离体培养及生根诱导 |
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| 引用本文： | 马燕,韩瑞超,臧德奎,郝日明.桂花幼胚的离体培养及生根诱导[J].山东农业科学,2013,45(3). |
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| 作者姓名： | 马燕 韩瑞超 臧德奎 郝日明 |
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| 作者单位： | 1. 山东农业大学林学院,山东泰安,271018
2. 南京农业大学园艺学院,江苏南京,210095 |
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| 基金项目： | 国家自然科学基金项目，山东农业大学青年科技创新基金项目 |
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| 摘 要： | 对3个桂花品种大花金桂、籽银桂、籽丹桂进行幼胚的离体培养,研究了不同消毒时间、基本培养基及生长调节剂组合对离体幼胚萌发的影响,以及不同培养条件和培养基配方对萌发苗生根的影响。结果表明,适合幼胚萌发的适宜培养基,大花金桂为:B5+1 mg/L 6-BA+3 mg/L GA;籽银桂:B5+2 mg/L 6-BA+2 mg/L GA;籽丹桂:B5+1.5 mg/L 6-BA+1 mg/L GA。生根适宜培养基为:1/2 MS+2 mg/L NAA。
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| 关 键 词： | 桂花 幼胚 离体培养 萌发 生根 |
Culture in vitro and Root Induction of Immature Embryos of Osmanthus fragrans |
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| Ma Yan
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Han RuiChao
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Zang DeKui
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Hao RiMing.Culture in vitro and Root Induction of Immature Embryos of Osmanthus fragrans[J].Shandong Agricultural Sciences,2013,45(3). |
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| Authors: | Ma Yan Han RuiChao Zang DeKui Hao RiMing |
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| Abstract: | Culture in vitro of immature embryos of three species of Osmanthus fragrans as 'Dahua Jingui','Zi Yin' and 'Zi Dangui'were carried out.The effects of different sterilization time,basic culture medium and different growth regulator on in vitro embryo germination,as well as the different culture conditions and medium on root induction were focused on.The results indicated that the optimal media for embryo germination of 'Dahua Jingui','Zi Yin'and 'Zi Dangui' was B5+1 mg/L 6-BA+3 mg/L GA,B5+2 mg/L 6-BA+2 mg/L GA and B5+1.5 mg/L 6-BA+1 mg/L GA respectively.The suitable medium for rooting was 1/2MS+2 mg/L NAA. |
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| Keywords: | Osmanthus fragrans Immature embryos Culture in vitro Germination Root induction |
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