| 鹅微卫星(TG)_n基序的克隆及序列比较分析 |
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| 引用本文： | 虞德兵,汪峰,洪坤月,杜文兴.鹅微卫星(TG)_n基序的克隆及序列比较分析[J].畜牧与兽医,2005,37(12):1-3. |
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| 作者姓名： | 虞德兵 汪峰 洪坤月 杜文兴 |
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| 作者单位： | 南京农业大学动物科技学院,江苏,南京,210095 |
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| 基金项目： | 国家科技攻关项目（2002BA514A-9-2-6）;江苏省农业三项工程项目（SX[2003]046）. |
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| 摘 要： | 以(TG)10重复基序设计引物对鹅基因组DNA进行PCR扩增,PCR产物经12%的非变性聚丙烯酰胺凝胶电泳后银染检测,共获得了7种基因型,4种等位基因;并且在各供试鹅品种上表现出多态性,可以实现遗传分类研究。对回收的不同条带进行纯化、克隆测序;测序结果显示各品种都有(TG)n基序存在,并且各个条带中重复数大于10。
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| 关 键 词： | 鹅 微卫星 克隆 |
| 文章编号： | 0529-5130（2005）12-0001-03 |
| 收稿时间： | 06 10 2005 12:00AM |
| 修稿时间： | 2005年6月10日 |
Cloning and comparative analysis of microsatellite motif (TG)n in goose |
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| YU De-bing,WANG Feng,HONG Kun-yue,DU Wen-xin.Cloning and comparative analysis of microsatellite motif (TG)n in goose[J].Animal Husbandry & Veterinary Medicine,2005,37(12):1-3. |
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| Authors: | YU De-bing WANG Feng HONG Kun-yue DU Wen-xin |
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| Institution: | College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | The microsatellite motif(TG)_(10)was synthesized and used as primer in PCR with goose DNA as template,and the PCR products were detected by 12% polyacrylamide gel electrophoresis(PAGE) and silver staining method.Seven genotypes and four allele fragments were amplified,and the goose breeds were identified according to their polymorphs.The various bands were purified and cloned and sequenced.The sequencing results indicated microsatellite motif(TG)_n existed in all goose breeds,and the numbers were above 10. |
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| Keywords: | microsatellite goose cloning |
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