| 林甸鸡和大骨鸡微卫星DNA标记遗传多样性的比较研究 |
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| 引用本文： | 白文林,尹荣焕,赵素君,曹亮,罗光彬,姜午旗.林甸鸡和大骨鸡微卫星DNA标记遗传多样性的比较研究[J].华北农学报,2005,20(6):24-27. |
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| 作者姓名： | 白文林 尹荣焕 赵素君 曹亮 罗光彬 姜午旗 |
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| 作者单位： | 沈阳农业大学,畜牧兽医学院,辽宁,沈阳,110161;四川省畜牧科学研究院,四川,成都,610066;黑龙江省农垦总局畜牧局,哈尔滨,150036 |
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| 基金项目： | 沈阳农业大学青年教师科研基金资助 |
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| 摘 要： | 为了从分子水平上揭示林甸鸡和大骨鸡的群体遗传结构，利用鸡基因组中5个微卫星标记，检测了林甸鸡和大骨鸡群体的遗传多样性。同时探讨了林甸鸡和大骨鸡在国内几个地方鸡种中的系统地位。结果表明，5个微卫星位点共检测到38个等位基因，其中ADL0146位点的等位基因数5个，为最少，而ADL0136和ADL0185两个位点的等位基因数9个，为最多；5个微卫星位点的平均等位基因数为7．6个。林甸鸡群体内比大骨鸡群体有更丰富的遗传多样性。聚类分析结果显示，林甸鸡和大骨鸡与边鸡、日照麻鸡及寿光鸡之间有比较密切的亲缘关系；而林甸鸡和大骨鸡与鲁西斗鸡的亲缘关系均较远。总体而言，该系统聚类结果与7个鸡种的分化与选育历史是一致的。
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| 关 键 词： | 鸡 微卫星标记 遗传多样性 聚类分析 |
| 文章编号： | 1000-7091（2005）06-0024-04 |
| 收稿时间： | 2005-06-12 |
| 修稿时间： | 2005-06-12 |
Comparative Study on Genetic Diversity of Lindian Chicken and Dagu Chicken Breeds Using Microsatellite DNA Markers |
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| BAI Wen-lin,YIN Rong-huan,ZHAO Su-jun,CAO Liang,LUO Guang-bin,JIANG Wu-qi.Comparative Study on Genetic Diversity of Lindian Chicken and Dagu Chicken Breeds Using Microsatellite DNA Markers[J].Acta Agriculturae Boreali-Sinica,2005,20(6):24-27. |
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| Authors: | BAI Wen-lin YIN Rong-huan ZHAO Su-jun CAO Liang LUO Guang-bin JIANG Wu-qi |
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| Institution: | 1. College of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang 110161, China; 2. Animal Science Research Academy of Sichuan, Chengdu 610066, China; 3. Animal Science Bureau, Land Reclamation General Bureau of Heilongjiang 150036, China |
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| Abstract: | For the sake of disclosure of population genetic construction of Lindian chicken and Dagu chicken breeds from molecular level,genetic diversity of Lindian chicken and Dagu chicken was assayed on the basis of 5 microsatellite DNA markers from the chicken gene bank,as well as,the phylogenitic status of Lindian chicken and Dagu chicken was discussed among several native chicken breeds.The results showed,altogether,38 alleles were detected in 5 microsatellite loci,and among them,the least alleles(5) were detected by ADL0146,and the most alleles(9) were detected by ADL0136 and ADL0185.The average alleles number was 7.6 from 5 microsatellite loci.Genetic diversity in Lindian chicken was more abundant than that of Dagu chicken.The cluster analysis indicated that the consanguinity was close relatively among Lindian chicken,Dagu chicken,Bian chicken,Rizhao Pockmarked chicken and Shouguang chicken breeds;however,the consanguinity was far relatively between Lindian chicken,Dagu chicken and Luxi fighting chicken breeds.As a while,the clustering diagram reflected well the evolutionary and breeding history of the 7 chicken breeds. |
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| Keywords: | Chicken Microsatellite markers Genetic diversity Cluster analysis |
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